摘要
病毒样颗粒(Virus-like particles,VLPs)是由一种或多种病毒结构蛋白组装成的的颗粒,具有与病毒颗粒相似的外部结构和抗原性,但不含病毒基因。以预防乙型肝炎病毒、乳头瘤病毒和戊型肝炎病毒感染为目的的三种VLP疫苗已被批准在人体应用。可采用细菌、酵母、昆虫细胞、哺乳动物细胞、植物细胞系统表达VLP抗原。表达的VLP抗原可经裂解、粗提、浓縮和精制四个基本步骤纯化。解聚/重组装处理可能增强VLP抗原的效力和稳定性。在研制和生产VLP疫苗的过程中要对VLP抗原的品质、含量、效力和纯度进行检测。
Virus-like particles （ VLPs） are particles formed by one or several structural proteins from viruses , with similar outside structure and antigenicity of the native viruses , containing no virus genome .Three VLP based human vaccines have been licensed for preventing infections of hepatitis B iv rus, human papilloma virus and hepatitis E virus, respectively .VLP antigen can be produced in bacteria , yeasts, insect cells, mammalian cells and plant cells .The produced VLP are purified through four stepes including lysis , extraction, concentration and refinement .The potency and stability of the VLP antigens could be improved by treatment of disassembl/reassembly .The related detections have been performed to monitor the iden-tity, quality, potency and purity of the VLP antigens in the process of development and mamufacture .
出处
《微生物学免疫学进展》
2014年第1期1-8,共8页
Progress In Microbiology and Immunology
关键词
病毒样颗粒
疫苗
表达
纯化
组装
检测
Virus like particles （VLPs ）
Vaccine
Expression
Purification
Assembly
Detection
作者简介
于永利，教授，博士生导师，主要从事疫苗和疫苗佐剂的基础和应用研究。通讯作者：于永利，E—mail：ylyu@mail．jlu．edu．cn
