摘要
目的观察免疫细胞因子在糖尿病周围神经病变(DPN)模型大鼠血清中的表达。方法实验大鼠分为正常对照组、糖尿病组和DPN组,每组各12只,腹腔注射STZ制备糖尿病大鼠模型。采用特殊染色及透射电镜法观察各组坐骨神经的组织病理学改变。ELISA法检测各组血清TNF-α、IL-1β及IL-6的表达,并分析其与DPN的相关性。结果组织染色及电镜结果显示,DPN大鼠组织病理学表现符合DPN表现。DPN组TNF-α[(1847.25±289.43)pg/ml]和IL-1β[(171.13±54.98)pg/ml]表达高于糖尿病组[(1430.58±255.56),(143.23±47.05)pg/ml,(P<0.05)],而IL-6[(989.45±200.48)pg/ml]表达水平与糖尿病组[(914.23±165.45)pg/ml]比较差异无统计学意义(P>0.05)。Pearson相关性分析发现,神经传导速度(NCV)与TNF-α呈直线负相关(r=—0.85,P=0.001)。结论细胞因子TNF-α及IL-1β在DPN大鼠血清中表达升高,且TNF-α是DPN发病的重要危险因素。
[Abstract] Objective To investigate the expression of immunocytokines in the serum of DPN （diabetic peripheral neuropathy） model rats, and the relationship between the cytokines and DPN. Methods The experimental rats were divided into three groups （n= 12）.. normal control group（NC）, diabetic rats group （DM） and DPN rats group. Rats model of diabetes mellitus was established by intraperitoneal injection of streptozotocin （STZ）. It was observed each group histopathological changes of sciatic nerve using specific stain and transmission electron microscopy, and detected each group the expression of TNF-α, IL-1β and IL-6 in serum of rats by ELISA, then analyzed the correlation between them and DPN. Results The morphologic features of sciatic nerve in rats with DPN consisted with pathological changes of DPN. Compared with DM group, the level of TNF-α（1847.25 ＋289.43） pg/ml and IL-113（171.13! 54.98）pg/ml in the group of DPN showed an obviously statistical increase （P〈 0.05） ; There were not significantly differences in the serum levels of IL-6 between DM group and DPN group （P 〈0. 05）. Pearson correlation showed that there was negative correlation between nerve conduction velocity and TNF-α（r=-0. 85,P=0. 001）. Conclusion The expressions of cytokines TNF-α and IL-113 are significantly increased in serum of DPN rats, and TNF-α is an important risk factor for DPN.
出处
《中国糖尿病杂志》
CAS
CSCD
北大核心
2014年第3期264-267,共4页
Chinese Journal of Diabetes
基金
上海市金山区卫生局科研项目(2011-07)
关键词
糖尿病周围神经病变
细胞因子
糖尿病
2型
肿瘤坏死因子-Α
Diabetic peripheral neuropathy
Cytokine
Diabetes mellitus, type 2
Tumor necrosisfactor-α（TNF-α）
作者简介
通信作者：施晓红，E-mail：cyhl973131@163．com
