摘要
将紫甘薯的茎段或茎尖作为外植体,培养在添加各种激素配比的培养基上,研究紫甘薯组培快繁影响因素,筛选培养所需最佳初代、增殖和生根的培养基及培养条件。结果表明:在外植体分化时的初代培养基MS+BA2.0mg/L,增殖培养基最佳配方为MS+BA1.0mg/L+NAAO.1~0.2mg/L,适合生根的培养基为1/2MS+NAA0.1mg/L+IAA0.1mg/L。
The use of sweet potato stem segments or shoot tips of purple as explants were cultured in medium proportions of various hormones, studying influencing factors of tissue culture of purple sweet potato, screening medium and culture conditions for training required for the best first generation, proliferation and rooting of. The results showed that: when the explant differentiation initial medium was MS + BA 2.0 mg/L, the best formula proliferation medium was MS+ BA 1.0 mg/L+ NAA 0.1-0.2 mg/L, suitable rooting medium was 1/2MS+ NAA 0.1 rag/L+ IAA 0.1 mg/L.
出处
《中国农学通报》
CSCD
2014年第4期96-100,共5页
Chinese Agricultural Science Bulletin
基金
玉溪市农业科学院科研项目"紫甘薯组织培养快繁技术"(nky2010029)
关键词
紫甘薯
组织培养
外植体
培养基
purple sweet potato
tissue culture
explants
medium
作者简介
张军云,男,1973年出生,高级农艺师,农业推广硕士,主要从事农业技术推广和植物组织培养工作。通信地址:653100云南省玉溪市红塔大道18号玉溪市农业科学院,E—mail:yuhebio@163.com。
通讯作者:张钟,男,1969年出生,云南玉溪人,高级工程师,本科,主要从事农业技术推广。通信地址:653100云南省玉溪市红塔大道18号玉溪市农业科学院,Tel:0877-2066427,E-mail:y_zhong@yxag.gov.cn。