摘要
本试验旨在初步探究并比较安琪与乐斯福两种不同来源酵母对奶牛瘤胃发酵特性的作用,预测其对奶牛生产性能可能的影响,从而为研究酵母在反刍家畜生产中营养生理调控提供理论依据。采用单因子区组试验设计,两种活性酵母均设置4个添加水平(0、0.025%、0.050%、0.075%),分别以玉米秸秆和水稻秸秆为底物,采集奶牛瘤胃液进行48h的体外发酵试验。结果表明,两种酵母对两种底物体外发酵产气量、产气参数、中性洗涤纤维消失率(NDFD)均无显著影响(P>0.05),但提高了玉米秸秆(P<0.05)和水稻秸秆(P<0.01)的DMD;添加两种酵母对玉米秸秆体外发酵液pH值及NH3-N浓度均没有表现出显著性影响(P>0.05),但均显著降低了水稻秸秆体外发酵液pH值及NH3-N浓度(P<0.05);乐斯福酵母对两种底物DMD的提高作用均显著高于安琪酵母(P<0.05)。通过整体比较可以推断,乐斯福酵母的添加效果要明显优于安琪酵母,且以0.075%为最佳添加水平。
The objective of this trial was to preliminarily explore and compare the effects of two sources of commercial active yeast additive （Angel Yeast ＆ Lesaffre Yeast） on the characteristics of in vitro fermentation, to predict their potential influences on the production performance of dairy cows, and to provide the regulation of nutrition physiology with theoretical basis. The study was conducted as one-factor block experimental design, and four supplemental levels （0, 0.025%, 0.050%, and 0.075%） were set for each source of active yeast. By employing the method of in vitro fermentation （48 hours）, maize straw and rice straw were respectively used as the substrate. The result of this study suggests that the supplementation of active yeast did not exert a significant influence on the GP, indexes of LE model, and NDFD （P〉0.05）, but it elevated the DMD of maize straw （P〈0.05） and rice straw （P〈0.01）. The supplementation of active yeast exerted a non-significant influence on the pH and NH3-N of/n vitro fermentation of maize straw （P〉0.05）, but it significantly affected the pH and NH3-N of in vitro fermentation of rice straw （P〈0.05）. In the in vitro fermentation of both maize straw and rice straw, the treatment of Lesaffre Yeast significantly raised the DMD compared to the treatment of Angel Yeast （P〈0.05）. It can be inferred that the effects of Lesaffre Yeast were more desirable than those of Angel Yeast, and the optimum supplemental level of the former was 0.075%.
出处
《农业现代化研究》
CSCD
北大核心
2014年第2期218-224,共7页
Research of Agricultural Modernization
基金
国家自然科学基金“C3和C4植物中13C标记测定山羊内源氮和内源氨基酸的方法研究”(编号:31001024)
国家科技支撑计划课题“中南稻区复合生物循环技术集成与示范”(编号:2012BAD14B17)
中国科学院亚热带农业生态研究所青年人才领域项目“植物自然富集13C研究山羊温室气体排放与氮沉积的方法”(编号:ISACX-LYQY-QN-1105)
关键词
酵母
瘤胃
体外发酵
奶牛
active yeast
rumen
in vitro fermentation
dairy cows
作者简介
王祚（1988-），男，湖南安化人，硕士研究生，研究方向：动物营养与饲料科学.
通讯作者：周传社（1980-），博士，副研究员，研究方向：反刍动物营养与生理调控研究。
