摘要
为研究黄山贡菊中黄酮的提取纯化方法及其抗小鼠急性肝损伤作用,采用乙醇回流法从黄山贡菊中提取粗黄酮,聚酰胺柱层析纯化,真空冷冻干燥获得黄酮粉末。对以四氯化碳造模的急性肝损伤小鼠分别给予联苯双酯及不同剂量的贡菊黄酮灌胃,测定血清中GPT/ALT、GOT/AST活性,测定肝组织中SOD活性和丙二醛含量,HE染色观察肝组织病理形态学的改变。结果表明,黄山贡菊黄酮可以降低急性肝损伤小鼠血清中GPT/ALT、GOT/AST活性,提高肝组织的SOD活性,降低丙二醛含量,对减轻肝脏病理组织损伤有积极作用。
The objective of this study was to explore the extraction and purification technique of flavonoids from Chrysan- themum Morifolium cv. Gongju and to investigate the effect of flavonoids against acute liver injury of mice. The flavonoids were extracted with aqueous ethanol refluxing, purified by column chromatography of polyamide, and flavone powders were obtained by vacuum freeze-drying method. An acute liver injury model of mice induced by carbon tetraehloride was established. The model mice were given bifendate and flavonoids from C. gorifolium cv. Gongiu respectively. The GPT/ ALT and GOT/AST in blood serum,the activity of SOD and the content of MDA in liver were determined,and the mor- phology changes of hepatic tissue were observed by HE. The results showed that flavonoids from C. Morifolium cv. Gongju reduced the activity of GPT/ALT and GOT/AST in blood serum of acute liver injury of mice, increased the activity of SOD and decreased the content of MDA in hepatic tissue, and relieved the damage of liver pathological tissue.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2014年第2期183-187,214,共6页
Natural Product Research and Development
关键词
黄山贡菊
黄酮
四氯化碳
急性肝损伤
Chrysanthemum Morifolium cv. Gongju
flavonoids
carbon tetrachloride
acute liver injury
作者简介
通讯作者E-mail：swhx12@ahau．edu．cn
