摘要
本研究前期成功分离获得1株对水稻真菌病害具有较强抑制作用的枯草芽孢杆菌T429,为开发生防菌农药新剂型,利用喷雾干燥法对生防菌T429发酵液进行处理,以喷雾干燥后产物活菌量为主要指标,采用单因素和正交试验法,对影响喷雾干燥产物指标的主要因素进行优化。结果表明,最佳喷雾干燥条件为进风温度120℃、进样速度720 mL/h、雾化口径1 mm、雾化压力0.1 MPa、热风流量30 M3/h,可获得产物活菌量达8.61×109 cfu/g。该工艺稳定、可控,为以枯草芽孢杆菌为核心的微生物杀菌剂新剂型的研发提供了科学依据。
Bacillus subtilis T429 was confirmed to be highly bacteriostatic to rice fungus diseases in previous study. Fermentation broth of B. subtilis T429 was granulated by spray-drying technology to develop new bacterial pesticide formulation. Single factor test and experiment with four-factor and three-level orthogonal were carried out to optimize spray-drying condition. Results showed that the optimum conditions were inlet air temperature 120℃, the feed flow rate 720 mL/h, atomization caliber 1 mm, atomization pressure 0.1 MPa, hot air flow 30 M3/h. The number of viable cells of products reached to 8.61×109 cfu/g under the optimal conditions. These data helps development of new pesticide formulation of B. subtilis.
出处
《中国生物防治学报》
CSCD
北大核心
2014年第1期101-106,共6页
Chinese Journal of Biological Control
基金
江苏省科技支撑计划(BE2011356)
农业部"948"项目(2011-G4)
关键词
枯草芽孢杆菌
农药剂型
喷雾干燥
正交试验
Bacillus subtilis
pesticide formulation
spray drying
orthogonal experiment
作者简介
孟祥坤（1982-），男，硕士，助理研究员，E-mail：1002xin@163．com；
通信作者，博士，研究员，E-mail：liuyf@jaas．ac．cn。
