摘要
目的:利用中红外光谱对不同厂家的冬凌草片进行鉴别。方法:分析不同厂家的冬凌草片的红外光谱原始、一阶导数和二阶导数光谱,并通过计算光谱间的距离将其聚类分析。结果:通过比较发现相同厂家冬凌草片的原始光谱相似度很大,不同厂家的冬凌草的红外光谱相似度比较小。差异主要体现在二阶导数图谱的3 563 cm-1处的N-H伸缩振动吸收峰;1 158,1 117,1 080,1 037 cm-1处的C-O伸缩振动吸收峰;983,862,680,655 cm-1处的C=C的面外弯曲振动吸收峰,几处的峰形、峰位、峰强不同。并且采用聚类分析方法对不同厂家的冬凌草片进行聚类分析。结论:利用红外光谱的二阶导数光谱和聚类分析都可成功的鉴别4个不同厂家的冬凌草片。该方法具有快速、无损、绿色、直观的特点。
Objective： To identify Donglingcao tablets from different manufacturers by infrared spectroscopy. Method ： The infrared spectroscopy of different manufacturers were analyzed by the original spectra; first derivative and second derivative. And it is clustered by calculating the distance between the spectra. Result： The original spectrum of same manufacturers is very simliar, and the similarities of different manufacturers are relatively small. In the second derivative spectrum, the main difference is the N - H stretching vibration absorption at 3 563 cm-1, C- O stretching vibration absorption at 1 158, 1 117, 1 080, 1 037 em-1 and C = C outside the plane bending vibration absorption at 983, 862, 680, 655 cm-1 The intensity, shape and position of absorption peaks were obviously difference in all those places. The samples of different manufacturers were identified using clustering analysis. Conclusion： Using the infrared spectrum of the second derivative and clustering analysis can be successful in the identification of four manufacturers of Donglingcao tablets. This method is rapid, nondestructive, green and intuitive features.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第3期66-70,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
河南省重大公益科研项目(081100912500)
河南省杰出人才项目(084200510017)
河南中医学院博士科研基金项目(BSJJ2011-15)
关键词
冬凌草片
中红外光谱
不同厂家
二阶导数光谱
聚类分析
Donglingcao tablets
infrared spectroscopy
different manufacturers
second derivative
clustering analysis
作者简介
龚海燕，硕士，讲师，从事中药质量研究，Tel：0371-65575838，E-mail：ghy_mz@163．com
[通讯作者]白雁，教授，博士生导师，从事利用现代仪器分析方法对中药质量控制的研究，E-mail：ghy—mz@163．com
