摘要
目的：采用高效液相色谱法对枳术宽中胶囊的含量进行测定，以控制本品质量。方法：SUNTEK Kro—masil C18（4．6mm×250mm，5μm）；Dikma Kromasil C18（4．6mm×250mm，5μm）柱，以乙腈-十二烷基硫酸钠溶液梯度洗脱为流动相，流速为1ml Mmin；检测波长225nm。结果：辛弗林在进样量0．0808～1．616μg范围内与峰面积有良好线性关系，回归方程为：Y=-4．154＋2602．5X，r=0．9999，平均回收率为97．2％；RSD为1．4％（n=9）。结论：本方法简便准确、专属性强、测定结果重复性好，适用于本品的含量测定。
Objective： To determine the content of synephrine in zhizhukuanzhong capsules by HPLC （high performance liquid chromatography） method to control the quality. Methods： Colunm SUNTEK Kromasil C ls （4.6 mm x 250 mm, 5 μm）;Dikma Kromasil C18 （4.6 mm×250 mm,5 μm）; Mobile phase： acetonitrile and potassium dihydrogen phosphate （ gradient elution） was set up. The flow rate was 1.0 mL . min^ - 1. Detection wavelength was 225 nm. Results： The linear relationship between sample volume and area of the peak in synephrine was good in the range of 0.0808 × 1. 616μg, with a correlation coefficient of 0.9999 and linear equation was Y= -4. 154 ＋2602.5X. The average recovery was 97.2% and relative the standard deviation（RSD） was 1.4% （n =9）. Conclusion： The method is simple, accurate and specific. The reduplication of the results is good, suitable for the quality control of this composite preparation.
出处
《山西职工医学院学报》
CAS
2013年第6期4-6,32,共4页
Journal of Shanxi Medical College for Continuing Education
作者简介
申国华，男，主管药师，从事中药、重金属及农药残留的检测工作
