摘要
目的探讨妊娠期糖尿病(gestational diabetes mellitus,GDM)与婴儿过敏性疾病的关系,并评价蛋白激酶Cζ(PKCζ)是否可作为婴儿早期预测过敏性疾病的指标。方法采用荧光定量PCR(RT-PCR)检测40例单胎足月妊娠正常孕妇及40例GDM孕妇外周血及80例足月新生儿脐血中PKCζ水平,并对这些婴儿进行随访,调查出生后6个月内过敏性疾病发生情况。结果(1)正常孕妇组与GDM孕妇组所分娩新生儿脐血PKCζ的表达值2^(-△△CT)分别为(0.0015±0.000571)及(0.0011±0.000323),两组比较差异有统计学意义(t=9.545,P=0.011);正常孕妇组与GDM孕妇组外周血PKCζ的表达值2^(-△△CT)分别为(0.0012±0.000511)及(0.0004±0.000205),两组比较差异有统计学意义(t=11.314,P<0.01);(2)正常孕妇组及GDM孕妇组婴儿过敏性疾病的发生率分别为15%和60%,比较有显著差异(χ~2=17.280,P<0.01);(3)GDM是婴儿过敏性疾病发生的高危暴露因素(0R=8.500,95%CI 2.905~24.879,P<0.01);(4)脐带血PKCζ的表达对婴儿过敏性疾病的诊断临界值为0.00128,敏感性35.5%,特异性34.7%,而外周血PKCζ的表达对婴儿过敏性疾病的诊断临界值为0.000995,敏感性12.3%,特异性32%。结论 GDM是过敏性疾病发生的高危暴露因素;脐血及孕妇外周血PKCζ对过敏性疾病均有预测作用。
Objective To explore the relationship between gestational diabetes（Gestational Diabetes Mellitus,GDM） and allergic diseases in infants and evaluate the predictive value of the protein kinase Cξ（PKCξ）.Methods Using real time PCR（RT-PCR） to detect PKCξ level in peripheral blood of 40 normal pregnant women and 40 pregnant women with GDM and PKCξ level in cord blood above 80 pregnant women. These infants were followed up and the allergic diaease incidence was investigated within six months after the birth.Results（1） The PKCξ expression value of umbilical cord blood of new born deliteried by normal pregnant women and GDM women are（0.0015±0.000571） and（0.0011±0.000323）,there exist significant difference（t=9.545,P=0.011）.The PKCξ expression value of peripheral blood of GDM pregnant women and normal pregnant women are（0.0004±0.00O_205） and（0.0012±0.000511）,which show significant difference（P0.01）;（2）The allergic diaease incidence of two groups are 15%（the normal group）,60%（the GDM group）,and there exist significant difference（x^2=17.280,P=0.0000.05）;（3）GDM is the exposure factors of allergic disease in infants（OR=8.500,95%CI：2.905-24.879,P=0.0000.05）;（4）The dignosis value of the expression of PKCξ in umbilical cord blood to allergic disease of infants is 0.00128,the sensitivity is 35.5%,the specificity is 34.7%.The dignosis value of the expression of PKCξ in peripheral blood to allergic disease of infants is 0.000995,the sensitivity is 12.3%,the specificity is 32%.Conclusions GDM is the exposure factors of allergic disease in infants.The protein kinase Cξ（PKCξ） in cord blood and maternal blood both can predict allergic disease.
出处
《妇产与遗传（电子版）》
2013年第3期22-26,共5页
Obstetrics-Gynecology and Genetics (Electronic Edition)
基金
广东省自然科学基金(S2011010003396)~~
关键词
糖尿病
妊娠
蛋白激酶Cζ
婴儿过敏性疾病
Diabetes Gestational Protein kinase C-epsilon Allergic diseases in infants
作者简介
通讯作者：何平，E—mail：heping-20088@163．com
