摘要
目的:采用近红外漫反射光谱技术,建立对道地山药的快速无损鉴别方法。方法:收集三个产地(河南、河北、广东)的山药饮片共90份,采集其近红外漫反射光谱。采用判别分析法建立定性鉴别模型,并考察不同数据预处理方法对模型预测结果的影响。结果:道地山药饮片与其他产地的山药饮片在原始光谱与主成分得分的空间分布中均表现出较大的差异性。所建定性判别模型中,经多元散射校正结合二阶导数及SG平滑处理的模型表现出最佳预测结果,其对校正集与验证集的判正率均为100%。结论:近红外漫反射光谱技术在道地药材的质量分析中具有可行性,可实现道地山药饮片的快速无损鉴别。
To investigate the feasibility of NIR spectroscopy in the discrimination of genuine Chinese Yam,90 samples from three origins were collected and their near infrared diffuse reflectance spectra were acquired. Discriminant analysis was employed to create qualitative model, and several data pre - processing methods were explored. The results showed that genuine Chinese Yam was greatly different from other samples according to raw spectra and score plot of PCA. The discriminant analysis model with raw spectra after pretreatment of mul-tiplicative scatter correction,2nd derivative and Savitzky-Golay filter smoothing, showed the best predicative ability （ the percentage of correctly identified varieties were both 100% for the calibration sample set and validation sample set ）. This research indicated that NIR diffuse reflection spectroscopy could be used for the rapid analysis of genuine Chinese Yam .
出处
《世界中医药》
CAS
2013年第11期1277-1279,共3页
World Chinese Medicine
基金
国家自然科学基金项目(编号:81303218)
省部级中药基础与新药研究重点实验室开放课题
关键词
道地山药
近红外漫反射光谱
判别分析法
鉴别
Genuine Chinese Yam
Near infrared diffuse reflectance spectroscopy
Discriminant analysis
Identification
作者简介
杜敏，研究方向：中药材质量的快速评价方法研究
通信作者：华国栋，副主任药师，TEL：（010）67638124，E-mail：zhjhsa@tom．com
通信作者：乔延江，教授，TEL：（010）84738621，E—mail：yjqiao@263．net；
