摘要
目的采用气相色谱法测定参苓白术散中樟脑、龙脑、乙酸龙脑酯的含量，并建立其质量标准。方法采用100％甲基聚硅氧烷为固定相的毛细管柱；程序升温：起始温度60℃，以5℃／min升到130℃，保持5min，续以20℃／min升到230℃，保持5min。结果樟脑平均回收率为99．87％，RSD=1．3％，进样量在0．00377～0．1508lag范围内呈良好的线性关系（r=0．9999）；龙脑平均回收率为100．71％，RSD=2．1％，进样量在0．002411～0．0964lag范围内呈良好的线性关系（r=0．9999）；乙酸龙脑酯平均回收率为101．95％，RSD=1．3％，进样量在0．00302～0．1211μg范围内呈良好的线性关系（r=0．9999）。结论本方法操作简便，结果可靠、准确，能有效控制参苓白术散中砂仁的质量。
Objective To establish a GC method for simultaneous determine of camphor, borneol and bornyl acetate in Shenling Baizhu Powder. Methods Capillary column was used with 100% methyl polysiloxane as stationary phase. The temperature-programmed was as follows： starting temperature of 60 ℃, 5 ℃/min, rose to 130 ℃, kept for 5 minutes, followed by 20 ℃/min up to 230 ℃, and kept for 5 minutes. Results The average recovery rate of camphor was 99.87%, RSD was 1.3%, and good linear relationship was showed in the range of 0.003 77-0.150 8 μg （r =0.999 9）. The average recovery of borneol was 100.71%, RSD was 2.1%, and good linear relationship was showed in the range of 0.002 411-0.0964 μg （r=0.999 9）. The average recovery rate of bornyl acetate was 101.95%, RSD was 1.3%, and good linear relationship was showed in the range of 0.00302-0.121 1 μg （r=0.999 9）. Conclusion The method is simple, reliable, accurate, and can effectively control the quality of Amomi Fructus in Shenling Baizhu Powder.
出处
《中国中医药信息杂志》
CAS
CSCD
2013年第12期56-58,共3页
Chinese Journal of Information on Traditional Chinese Medicine
作者简介
通讯作者：吴彬，Tel：010-83979468，E-mail：wubin@bjdagov．cn.
