摘要
目的:基于血清药物化学方法,对龙眼叶乙酸乙酯部位进行入血成分分析,探讨龙眼叶的药效物质基础。方法:采用UPLC-Q-TOF-MS/MS高分辨液质联用法,对乙酸乙酯部位及给药后大鼠血清样品进行分离后在负离子检测模式下进行总离子检测、二级碎片离子扫描,初步鉴定龙眼叶提取物在大鼠血中原型成分及代谢成分。结果:从含药血清中初步鉴定了共17个药源性成分,其中有1个原型成分曾从龙眼叶的提取物中得到分离,其余多为原型成分的苷元代谢产物。结论:初步确定了没食子酸乙酯、槲皮素、槲皮苷、木犀草素等成分可能为龙眼叶降血糖活性的物质基础。
Objective: To analyze the blood-transition constituents of ethyl acetate fraction of leaves from Dimocarpus longan based on the serum pharmacochemical method, and to investigate the pharmacological material basis of leaves from Dimocarpus longan.Methods: Using UPLC-Q-TOF-MS/MS high resolution liquid/mass spectrometry, the ethyl acetate fraction and serum samples of rats after administration were separated.Total ion detection and secondary fragment ion scanning were carried out under the negative ion detection mode to preliminarily identify the prototype components and metabolic components of extract from Dimocarpus longan leaves in the blood of rats.Results: A total of 17 drug-derived components were identified from the drug-containing serum, among which one prototype component was isolated from the extract of Dimocarpus longan leaves, and most of the others were aglycones metabolites of the prototype components.Conclusion: It is preliminarily determined that ethyl gallate, quercetin, quercitrin, luteolin and other components may be part of the substance basis of hypoglycemic activity of leaves of Dimocarpus longan.
作者
梁洁
韦金玉
黄冬芳
麦嘉妮
胡珏
祁金丽
赵立春
LIANG Jie;WEI Jin-yu;HUANG Dong-fang;MAI Jia-ni;HU Jue;QI Jin-li;ZHAO Li-chun(College of Pharmacy,Guangxi University of Chinese Medicine/Guangxi Zhuang Yao Medicine Center of Engineering and Technology,Nanning 530200,China)
出处
《中药材》
CAS
北大核心
2021年第3期615-621,共7页
Journal of Chinese Medicinal Materials
基金
国家自然科学基金项目(81560691)
壮瑶药协同创新中心(桂教科研[2013]20号)
广西高等学校高水平创新团队及卓越学者计划项目(桂教师范[2019]52号)
广西中医药大学“岐黄工程”高层次人才团队培育项目(2018002)
广西高等学校千名中青年骨干教师培育计划(桂教人[2019]5号)
关键词
龙眼叶
入血成分
血清药物化学
含药血清
UPLC-Q-TOF-MS/MS
Leaves of Dimocarpus longan Lour.
Absorbed compounds in the blood
Serum pharmacology
Drug-containing serum
UPLC-Q-TOF-MS/MS
作者简介
梁洁(1979-),女,博士,教授,研究方向:中药药效物质基础与质量控制,Tel:0771-3137585,E-mail:liangjie1101@126.com;通讯作者:赵立春,Tel:0771-4953513,E-mail:hyzlc@126.com。
