摘要
目的 建立保健食品中烟酰胺、维生素B1、维生素B2及维生素B6等4种水溶性维生素的高效液相色谱（HPLC）同时测定法.方法 样品经提取液提取、过滤后,以ZORBAX Eclipse XDB-C18化学键合硅胶为固定相,甲醇-1％（体积分数）冰乙酸溶液（含5.3 mmol/L己烷磺酸钠,pH2.8,体积比为25：75）为流动相,等度洗脱,紫外检测器于280nm波长下检测.结果 4种水溶性维生素在10～150 μg/L线性范围内均呈较好的线性关系,r＞0.999.该方法的检出限为2～5mg/100 g ·mL,平均回收率为80.9％～112.2％,相对标准偏差（RSD）为0.4％～3.6％.结论 高效液相色谱法快速简便,准确度和精密度均较高,适用于保健食品中多种水溶性维生素含量的同时测定.
Objective To develop an assay for simultaneous detection of 4 water-soluble vitamins （nicotinamide, vitamin B1, B2 and B6） by high performance liquid chromatography （HPLC）. Methods Samples were extracted, filtrated and loaded to HPLC. ZORBAX Eclipse XDB-C18 chemically bonded silica gel was used as stationary phase, methanoi-1 % （volume frac- tion） of glacial acetic acid solution （5.3mmol/L hexane sulfonate, pH2.8, volume ratio 25：75） was used as mobile phase un- der isoeratic elution; absorbance at 280nm was monitored by UV detector. Results all 4 water-s01uble vitamins showed good linear relationship in linear range of 10- 150btg/L, r2〉0. 999. The detection limit was 2- 5 mg/100 g % mL, average recovery efficiency was 80.9 %- 112.2 %, relative standard deviation （RSD） was 0.4 %- 3.6 %. Conclusion HPLC assay is quick and easy, with high accuracy and precision. It is suitable for simultaneous detection of multiple water--soluble vitamins in dietary supplements.
出处
《江苏预防医学》
CAS
2013年第6期28-30,共3页
Jiangsu Journal of Preventive Medicine
基金
"十二五"科教兴卫工程创新平台(ZX201109)
关键词
保健食品
水溶性维生素
高效液相色谱(HPLC)
同时测定
food supplement
water-soluble vitamin
high performance liquid chromatography （HPLC）
simultaneous detection
作者简介
杨润（1970-）,女,江苏无锡人,副主任技师,主要从事食品卫生理化检测工作.
通讯作者：滕小沛，E-mail：xiaomianzi@hotmail。com
