摘要
目的:研究分泌型卷曲相关蛋白1(secreted frizzled-related protein 1,SFRP1)基因启动子区与食管鳞癌的相关性.方法:选取食管鳞癌(esophageal squamous cell carcinoma,ESCC)患者22例为实验组,另同期选取食管良性病变者22例为对照组,提取术前外周血DNA,利用甲基化特异性PCR法(MSP)检测SFRP1基因启动子区域甲基化状态,并分析其与临床病理参数的相关性.结果:在22例食管鳞癌患者中其甲基化阳性例数为8例,甲基化阳性率为36.4%,在22例食管良性病变者中其甲基化阳性例数为2例,甲基化阳性率为9.1%(P<0.05).SFRP1基因甲基化与患者临床病理参数及癌胚抗原(carcino-embryonic antigen,CEA)水平无相关性(P>0.05).结论:血清SFRP1基因启动子区异常甲基化可能参与食管鳞癌的发生,有望成为食管鳞癌临床诊断的新型辅助指标.
AIM： To study the correlation between secreted frizzled-related protein I （SFRP1） gene promoter hypermethylation and clinicopathological fea- tures of esophageal squamous cell carcinoma （ESCC）. METHODS： Twenty-two patients with ESCC and 22 patients with benign esophageal diseases were included in this study. DNA was extracted from peripheral blood. Promoter methylation status of the SFRP1 gene was determined by methylation-specific polymerase chain reaction （MSP）, and its correlation with clinicopathologi- cal parameters of ESCC was analyzed. RESULTS： The positive rate of SFRP1 gene pro- moter methylation was significantly higher in ESCC patients than in controls （36.4% vs 9.1%, P 〈 0.05）. SFRP1 gene hypermethylation was not correlated with clinicopathological parameters or carcino-embryonic antigen levels in ESCC pa- tients （all P 〉 0.05）. CONCLUSION： SFRP1 gene promoter hyper- methylation may be involved in the occurrence of ESCC, and SFRP1 may be used as a new marker for ESCC.
出处
《世界华人消化杂志》
CAS
北大核心
2013年第31期3400-3404,共5页
World Chinese Journal of Digestology
基金
南京军区南京总医院院管基金资助项目
No.2011052~~
关键词
食管鳞癌
分泌型卷曲相关蛋白1
甲基化
Esophageal squamous cell carcinoma
Secreted frizzled-related protein 1
Methylation
作者简介
刘畅，在读博士，主治医师，主要从事消化系肿瘤的早期诊断和治疗以及分子流行病学的研究
通讯作者：汪芳裕，教授，主任医师，210002，江苏省南京市中山东路305号，中国人民解放军南京军区南京总医院消化内科wangf65@yahoo．com
