摘要
植被覆盖率是反映植株生长势的重要生理性状，在旱作地区尤为重要。图像处理技术能够快速有效地对苗期和孕穗期植被覆盖率进行量化分析。以28份山东小麦主栽品种和品系为材料，在240株m^-2和360株m-2密度下，连续2年测定了孕穗前不同发育阶段的植被覆盖率，并利用921个DArT标记和83个SSR标记分析了与植被覆盖率相关的遗传区段。结果表明，不同密度下，冬小麦植被覆盖率在越冬期、返青期和孕穗期存在显著差异，而拔节期基本一致。拔节期植被覆盖率与春季最高分蘖数、抽穗后群体叶面积指数、单位面积穗数和籽粒产量均呈显著正相关，r=0．73～0．76（P〈0．01），表明拔节期植被覆盖率可用于预测上述性状。共检测出12个遗传区段与植被覆盖率相关联，大部分区段直接参与调控苗期和孕穗期的生长势。10个遗传区段与已报道的苗期性状、产量性状及抗病位点一致，其中5BL、6AS和6BL染色体上携带的植被覆盖率相关遗传区段与已报道的苗期比叶面积和生物量等位点完全相同。建议将植被覆盖率作为生长势量化指标，用于育种选择和遗传研究。
Vegetative vigour is an important physiological trait and selection for greater seedling vigour is a goal of breeding programs, especially in rain-fed regions. This study aimed to identify the genetic variation of early vigour, determine the agronomic traits most closely associated with seedling growth, and detect the major gene-containing region of early vigour in winter wheat. Twenty-eight eultivars and advanced lines at two planting densities （240 plants m-2 and 360 plants m-2） were grown in Jinan during 2009-2010 and 2010-2011 cropping seasons, with randomized complete block design of three replications. Whole-genome association mapping was employed to identify the chromosome region controlling early vigour using 921 Diversity Array Technology （DART） and 83 SSR markers. Early vigour was evaluated with vegetative ground cover rate via implementation of photographic image analysis, whereby computer analysis was used to determine percentage ground cover. Significant differences of ground cover rate between two planting densities were detected in pre-winter period, greening-up and booting stages, but not in early stem elongation stage. Ground cover rate in stem elongation stage was significantly and positively associated with maximum tiller number （r = 0.76, P 〈 0.01）, leaf area index （r = 0.74, P 〈 0.01）, spike number （r = 0.73, P 〈 0.01）, and grain yield （r = 0.73, P 〈 0.01）. Twelve gene-containing regions for vegetative ground cover rate were detected in two seasons. Most of the regions conditioning the vegetative ground cover rate were not affected by the developmental stages. Ten gene-containing regions identified were consistent with previously reported QTLs for seedling traits, grain yield and disease resistance. Three regions on 5BL, 6AS, and 6BL were the same as previously reported loci for seedling traits. Therefore, there is sufficient genetic variation to increase early vigour in winter wheat, and early vigour could be quickly measured through digital image analysis.
出处
《作物学报》
CAS
CSCD
北大核心
2013年第11期1935-1943,共9页
Acta Agronomica Sinica
基金
国家自然科学基金项目(31161140346和31201207)
旱区作物逆境生物学国家重点实验室(西北农林科技大学)开放课题资助
关键词
普通小麦
植被覆盖率
关联分析
遗传区段
Common wheat
Vegetative ground cover rate
Association study
Gene-containing region
作者简介
第一作者联系方式：E—mail：xiaoyonggui@caas．cn，Tel：010—82108745
通讯作者（Correspondingauthor）：何中虎，E-mail：zhhecaas@163．com.
