摘要
用酶联免疫测定法研究了休眠期山药珠芽内源激素二氢玉米素(DHZR)、玉米素(ZR)、吲哚乙酸(IAA)、脱落酸(ABA)和赤霉酸(GA3)含量的变化规律,并研究了多效唑(PP333)对不同休眠程度的山药珠芽破除休眠的效果及对休眠期珠芽内源GA3含量的影响。结果表明,休眠期DHZR含量低变化小;ZR含量较DHZR含量高,休眠期含量升高,尤其在第80天时出现明显上升;ABA含量在休眠前期70 d缓慢下降,第80天后含量较稳定;IAA和GA3的含量都是在前70 d先降后升,第80天后,IAA含量变化小,而GA3含量下降直到珠芽发芽才升高。PP333可以促进贮藏80 d后的珠芽发芽,加快内源激素GA3含量的下降过程,促进珠芽发芽。由此可见,山药珠芽发芽需要GA3含量降低和ZR含量升高,PP333使珠芽内源激素GA3含量下降速度加快,缩短休眠期。
The content of endogenous hormone including of dihydrozeatin （DHZR）,zeatin （ZR）,indoleacetic acid （IAA）,abscisic acid （ABA） and gibberellinic acid （GA3） in dormant bulbils was tested every 10 days with Enzyme Linked Immunosorbent Assay （ELISA）.The germination percentage of bulbils treated with PP333 solution （10 mg/L） after stored 20,40,60 and 80 d was tested,and the content of endogenous hormone GA3 in bulbils treated continually with PP333solution （10 mg/L） was also tested,in order to study the variation of endogenous hormone in dormant yam（Dioscorea opposita）bulbils and response of dormant bulbils to PP333.The results showed that the concentration of DHZR in dormant bulbils was low and varied gently,while the concentration of ZR was higher than DHZR and it rose with bulbils becoming germinating and rose rapidly after 80 days.The concentration of ABA continued to reduce in the initial 70 d,and it was stable after 80 d.Both the concentrations of IAA and GA3 rose firstly then reduced in the initial 70 d,then since the bulbils have been stored for 80 d,the concentration of IAA changed little,while the concentration of GA3 kept to reducing until the bulbils germinated.PP333 solution could significantly promote bulbils germination after stored 80 d and the concentration of GA3 reduced quickly in this process.This study indicated that bulbils germination resulted from the reduction of GA3 content and the increase of ZR content,and PP333 accelerated the course of reduction of GA3 content,so PP333 promoted yam bulbils germination.
出处
《西南农业学报》
CSCD
北大核心
2013年第5期1996-2000,共5页
Southwest China Journal of Agricultural Sciences
基金
云南省科技厅项目(ZY2002-2)
云南省教育厅项目(2011C176)
云南省种业专项[云财农(2012)172号]
关键词
山药
珠芽
内源激素
多效唑
休眠
Dioscorea opposita
Bulbil
Endogenous hormone
PP333
Dormancy-break
作者简介
龙雯虹,女,博士,副教授,主要从事薯蓣资源评价与利用研究,E-mail：lwh57@sina.com
为通讯作者，E—mail：ylrghc@126．com。
