摘要
目的制定气管炎丸的质量标准。方法对方中麻黄、化橘红、党参进行薄层色谱（TLC）鉴别；采用高效液相色谱（HPLC）法测定柚皮苷的含量，色谱柱为C18柱（4．6mm×150mm，5μm）；以甲醇-水（40：60）为流动相；检测波长为283nm，流速1mL·min^-1。结果薄层色谱鉴别中，供试品在与对照药材色谱相应的位置上，显示相同颜色的荧光斑点，斑点清晰，分离效果好，阴性未见干扰；HPLC法含量测定中，柚皮苷在0．1952～1．9520μg（r=0．9999，n=7）范围内呈良好的线性关系，平均加样回收率为100．82％（RSD为0．87％）。结论所建立方法简便、准确、专属性强、重复性好，能有效控制气管炎丸的质量。
Objective To establish a quality control method of qiguanyan pill Methods TLC method was used to differentiate the ephedrine, codonopsis and citri grandis exocarpium. HPLC method was employed to determine naringin content. A C,s column （4.6 mm×150 mm,5 μm） was used with methanol and water （40 ： 60） as a mobile phase. The flow rate was 1 mL · min^-1 , detection wavelength 283 nm. Results Samples and controls had the same color fluorescence spots at the same position. Spots were well isolated. Linear range was 0. 195 2-1. 952 0 μg （r= 0. 999 9, n = 7）. Average recovery was 100.82% （RSD = 0. 87%, n = 6 ） . Conclusion The established method was sensitive, accurate, repeatable and specific for determination of naringin. It can be used for quality control of qiguanyan pill.
出处
《医药导报》
CAS
北大核心
2013年第10期1352-1355,共4页
Herald of Medicine
作者简介
作者简介邓永强（1961-），男，陕西扶风人，副主任药师，学士，主要从事医院药学工作。电话：0931—8993086，E—mail：dyq6698@163．com。
