摘要
目的：探讨CyclinE和p27在喉鳞状细胞癌（1aryngealsquamouscellcarcinoma，LSCC）中的表达水平及临床意义。方法：应用免疫组织化学EliVision染色法检测160例LSCC和30例正常喉黏膜组织（normallaryngealtissues，NLT）中CyclinE和p27的表达水平，并分析CyelinE和p27表达水平与LSCC临床病理参数之间的关系，以及CyclinE和p27表达之间的关系。结果：LSCC中CyclinE和p27阳性率分别为64．37％和40．0％，NLT中CyclinE和p27阳性率分别为16．67％和60．0％，差异均有统计学意义（P〈0．05～P〈0．01）；LSCC中CyclinE和p27的表达在肿瘤分化程度、淋巴结转移和临床分期间差异均有统计学意义（P〈0．05～P〈0．01），而在年龄、性别、肿瘤部位及肿瘤直径间差异均无统计学意义（P〉0．05）。CyclinE和p27呈负相关关系（P〈0．05）。结论：CyclinE和p27在LSCC和NLT的差异表达可能是LSCC发生和侵袭转移的分子基础，联合检测CyclinE和p27对判断LSCC预后有重意义。
Objective：To investigate the expression level of CyclinE and p27 in laryngeal squamous cell carcinoma（LSCC） and their clinical significance. Methods：The expression of CyclinE and p27 in 160 LSCC and 30 normal laryngeal tissues （NLT） were detected by EliVision immunohistochemical method. The relationship between the expression of CyclinE and p27 and clinicopathological parameters of LSCC was analyzed, the relationship between the expression of CyclinE and p27 was analysed. Results：The positive rates of CyclinE and p27 expression in LSCC and NLT were 64.37% ＆ 40.0% and 16.67% ＆ 60.0% ,respectively,the difference of which was statistical significance（ P 〈 0.05 to P 〈 0.01 ）. The expression differences of CyclinE and p27 in differentiation, lymph node metastasis and pTNM stage of LSCC were statistical significance （P 〈 0.05 to P 〈 0.01 ）, the expression differences of CyclinE and p27 in age, gender, anatomic location and diameter of tumor were not statistical significance （ P 〉 0.05 ）, the expression of CyclinE and p27 was negative relation （P 〈 0.05 ）. Conclusions：The CyclinE and p27 expression difference maybe a molecular basis of development and invasion/metastasis of LSCC. The detection of CyclinE and p27 expression has important significance in predicting the prognosis of patients with LSCC.
出处
《蚌埠医学院学报》
CAS
2013年第10期1285-1287,1290,共4页
Journal of Bengbu Medical College
作者简介
张明洁（1980-），女，主治医师．
[通信作者]刘业海，主任医师，教授．E-mail：yehailiu2003@yahoo.com．cn
