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组织芯片检测p16蛋白在不同胃组织中的表达意义 被引量：5

Significance of p16 Protein in Different Gastric Tissues De-tected by Tissue Array
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摘要 [目的]分析胃癌相关差异表达蛋白p16在不同胃组织中的表达意义,为临床早期发现胃癌及评估胃癌患者预后提供有价值的资料。[方法]采用免疫组织化学染色,检测胃组织芯片(包括正常胃黏膜、癌旁、非典型增生、胃癌及淋巴结转移癌组织)中p16蛋白的表达,分析其在胃癌组织中表达与临床病理特征的关系。[结果]p16蛋白在正常胃黏膜、癌旁、非典型增生和胃癌组织中的表达率分别为76.47%(26/34)、79.59%(39/49)、34.62%(9/26)和8.64%(7/81);p16蛋白在胃癌组织中阳性表达率较正常胃黏膜、癌旁组织和非典型增生组织降低(P<0.01),而非典型增生组织中阳性表达率较正常胃黏膜和癌旁组织降低(P<0.05);p16蛋白表达与胃癌患者年龄、肿块大小和淋巴结转移有关(P<0.05)。[结论]p16蛋白表达与胃癌的发生发展、患者年龄、肿块大小及淋巴结转移有关。 [Purpose] To analysis the expression of gastric cancer related differential expression protein p16 in various gastric tissues,and to provide valuable data for clinical early detection and prognosis evaluation.[Methods] The expression of p16 protein was analyzed by immunohisto-chemistry in gastric tissue array including normal gastric mucosa,paracancerous tissue,atypical hyperplasia tissue,gastric cancer tissue and metastatic lymph node.The relationship between ex-pression of p16 and clinicopathologic features in gastric cancer were analyzed.[Results] The ex-pression rate of p16 protein was 76.47%（26/34）,79.59%（39/49）,34.62%（9/26） and 8.64%（7/81） respectively in normal gastric mucosa tissue,paracancerous tissue,atypical hyperplasia tissue and gastric cancer tissue.The positive rate of p16 protein in gastric cancer tissue was lower than that in normal gastric mucosa tissue,paracancerous tissue and atypical hyperplasia tissue（P 0.01）.The positive rate in atypical hyperplasia tissue was lower than that in normal gastric mucosa tissue and paracancerous tissue（P0.05）.The expression of p16 protein related to age,size of tumor and lymph node metastasis of gastric cancer（P0.05）.[Conclusion] The expression of p16 protein re-lates to cancer progress,age,size of tumor and lymph node metastasis of gastric cancer.
作者 郭玉凤 张志伟 贺修胜 汤国辉 谢妮 杨科
机构地区 南华大学医学院肿瘤研究所 湖南文理学院体育学院 南华大学肿瘤细胞与分子病理学湖南省高校重点实验室 南华大学附属南华医院
出处 《肿瘤学杂志》 CAS 2012年第10期746-749,共4页 Journal of Chinese Oncology
基金 湖南省高校创新平台开放基金(10K052) 湖南省研究生科研创新项目(CX2010B380) 湖南省大学生研究性学习和创新性实验计划项目(CXSY-SJ-09007 CXSY-SJ-10192) 湖南省教育厅科研项目(11C1112) 衡阳市科技局科研项目(2011kj52 2011kj53)
关键词 胃肿瘤 P16蛋白 临床病理学 免疫组织化学 gastric neoplasms p16 protein clinicopathology immunohistochemistry
分类号 R735.2 [医药卫生—肿瘤]
作者简介 通讯作者:张志伟,副教授,硕士生导师,博士,南华大学肿瘤研究所,湖南省衡阳市常胜西路28号(421001),E-mail:zhangzhiweichina@yahoo.com.cn;通讯作者:贺修胜,博士生导师,博士,南华大学肿瘤研究所,湖南省衡阳市常胜西路28(421001),E-mail:hexiusheng@hotmail.com。
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参考文献21

  • 1Wroblewski LE,Peek RM Jr,Wilson KT. Helicobacter py- lori and gastric cancer: factors that modulate disease risk [J].Clin Microbiol Rev,2010,23(4):713-739.
  • 2Nguyen LT,Uchida T,Murakami K,et al. Helicobacter pylori virulence and the diversity of gastric cancer in Asia [J]. J Med Microbiol,2008,57(Pt 12):1445-1453.
  • 3Fuccio L,Zagari RM,Eusebi LH,et al. Meta-analysis: can Helicobacter pylori eradication treatment reduce the risk for gastric cancer? [J]. Ann Intern Med , 2009 ,151(2):121- 128.
  • 4Paoletti X,Oba K,Burzykowski T,et al. Benefit of adju- vant chemotherapy for resectable gastric cancer: a meta- analysis [J]. JAMA, 2010,303(17):1729-1737.
  • 5Islami F, Sheikhattari P, Ren JS, et al. Gastric atrophy and risk of oesophageal cancer and gastric cardia adenocarci- noma--a systematic review and meta-analysis [J]. Ann Oncol, 2011,22(4):754-760.
  • 6李艳兰,贺修胜,陈彦,容映晖,罗桥,宋颖,龙志峰,苏坚.胃腺癌组织蛋白质双向电泳图谱分析[J].南华大学学报（医学版）,2004,32(3):278-282. 被引量：5
  • 7陈彦,贺修胜,容映晖,罗桥,宋颖,龙志峰,苏坚,李艳兰.胃癌组织及癌旁组织蛋白质双向电泳图谱的差异分析[J].中国医师杂志,2004,6(4):442-444. 被引量：1
  • 8陈苏琼.LCM结合定量蛋白质组学技术筛选胃癌组织差异表达蛋白质[D].衡阳:南华大学,2011.
  • 9张志伟,汤国辉,赵强,刘重元,吴勇军,贺修胜.AKT-p27^(Kip1)-Cyclin E在胃癌组织中的表达及意义[J].世界华人消化杂志,2011,19(21):2233-2240. 被引量：12
  • 10Dhalla F,da Silva SP, Lucas M,et al. Review of gastric cancer risk factors in patients with common variable im- munodeficiency disorders,resulting in a proposal for a surveillance programme [J]. Clin Exp Immunol,2011,165 (1):1-7.

二级参考文献26

  • 1Corbett JM,Dunn MJ,Posch A,et al.Positional reproducibility of protein spots in two -dimensional polyacrylamide gel electrophoresis using immobilized pH gradients isoelectric focusing in the first dimension:An interlaboratory comparison[J].Electrophoresis
  • 2Hirano T,Fujioka K,Franzen B,et al.Relationship between TAO1 and TAO2 polypeptides associated with gastric adenocarcinoma and histocytological features[J].Br J cancer,1997,75(5):978-985
  • 3Sekido Y,Fong KM,Minna JD.Progress in understanding the molecular pathogenesis of human gastric adenocarcinoma[J].Biochim Biophys Acta,1998,1378(1):21-59
  • 4Araki N,Morimasa T,Sakai T,et al.Comparative analysis of brain proteins from P53-decificient mice by two-dimensional electrophoresis[J].Electrophoresis,2000,21(9):1880-1889
  • 5Gorg A,Obermaier C,Boguth G,et al.The current state of two-dimensional electrophoresis with immobilized pH gradients[J].Electrophoresis,2000,21(6):1037-1053
  • 6Ong SE,Foster LJ,Mann M.Mass spectrometric-based approaches in quantitative proteomics[J].Methods,2003,29(2):124-130
  • 7Petricoin EE,Paweletz CP,Liotta LA.Clinical applications of proteomics:proteomic pattern diagnostics[J].J Mammary Gland Biol Neoplasia,2002,7(4):433-440
  • 8Sinha SK,Zachariah S,Quinones HI,et al.Role of TRAF3 and -6 in the Activation of the NF-kappa B and JNK Pathways by X-linked Ectodermal Dysplasia Receptor[J].J Biol Chem,2002,277(47):44953-44961
  • 9Lopez AD. Counting the dead in China: measuring tobacco's impact in the developing world [J]. BMJ, 1998, 317(7170): 1399-1400.
  • 10Sekido Y, Fong KM, Minna JD. Progress in understanding the molecular pathogenesis of human gastric adenocarcinoma [J]. Biochim Biophys Acta, 1998, 1378(1): 21-59.

共引文献15

同被引文献49

  • 1Jin-Hong Luo,Jun Zhou,Yong Gao.Correlation between periostin and SNCG and esophageal cancer invasion,infiltration and apoptosis[J].Asian Pacific Journal of Tropical Medicine,2013,6(7):516-519. 被引量：6
  • 2Cappellani A, Zanghi A, Di Vita M, Zanet E, Veroux P, Cacopardo B, Cavallaro A, Piccolo G, Lo Menzo E, Murabito P, Berretta M. Clinical and biological markers in gastric cancer: update and perspec- tives. Front Biosci (Schol Ed) 2010; 2:403-412 [PMID: 20036956].
  • 3Bai Z, Ye Y, Liang B, Xu F, Zhang H, Zhang Y, Peng J, Shen D, Cui Z, Zhang Z, Wang S. Proteomics-based identification of a group of apoptosis-related pro- teins and biomarkers in gastric cancer. Int J Oncol 2011; 38:375-383 [PMID: 21165559 DOI: 10.3892/ ijo.2010.873].
  • 4Bosco R, Melloni E, Celeghini C, Rimondi E, Vac- carezza M, Zauli G. Fine tuning of protein kinase C (PKC) isoforms in cancer: shortening the distance from the laboratory to the bedside. Mini Rev Med Chem 2011; 11:185-199 [PMID: 21534929].
  • 5Redden JM, Le AV, Singh A, Federkiewicz K, Smith S, Dodge-Kafka KL. Spatiotemporal regulation of PKC via interactions with AKAP7 isoforms. Biochem J 2012; 446:301-309 [PMID: 22670899 DOh 10.1042/ BJ20120366].
  • 6Farah CA, Sossin WS. The role of C2 domains in PKC signaling. Adv Exp Med Biol 2012; 740:663-683 [PMID: 22453964 DOI: 10.1007/978-94-007-2888-2_2 9].
  • 7Chen T, Cao L, Dong W, Luo P, Liu W, Qu Y, Fei Z. Protective effects of mGluR5 positive modulators against traumatic neuronal injury through PKC- dependent activation of MEK/ERK pathway. Neu- rochem Res 2012; 37:983-990 [PMID: 22228200 DOh 10.1007/sl1064-011-0691-z].
  • 8Lai KC, Huang AC, Hsu SC, Kuo CL, Yang JS, Wu SH, Chung JG. Benzyl isothiocyanate (BITC) inhib- its migration and invasion of human colon cancer HT29 cells by inhibiting matrix metalloprotein- ase-2/-9 and urokinase plasminogen (uPA) through PKC and MAPK signaling pathway. J Agric Food Chem 2010; 58:2935-2942 [PMID: 20136087 DOI: 10.1021/jf9036694].
  • 9Li L, She H, Yue S, Feng D, Luo Z. Vasoactive intestinal peptide induces surfactant protein A expression in ATII cells through activation of PKC/ c-Fos pathway. Peptides 2010; 31:2046-2051 [PMID: 20688121 DOI: 10.1016/j.peptides.2010.07.017].
  • 10Reddy TR, Li C, Guo X, Myrvang HK, Fischer PM, Dekker LV. Design, synthesis, and structure- activity relationship exploration of 1-substituted 4-aroyl-3-hydroxy-5-phenyl-lH-pyrrol-2(5H)-one analogues as inhibitors of the annexin A2-S100A10 protein interaction. J Med Chem 2011; 54:2080-2094 [PMID: 21375334 DOI: 10.1021/jm101212e].

引证文献5

二级引证文献17

肿瘤学杂志
2012年 第10期

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