摘要
目的:采用烟熏联合气管内脂多糖(lipopolysaccharide,LPS)滴入方法建立慢性阻塞性肺疾病(chronic obstructive pulmo-nary disease,COPD)大鼠模型,探讨淫羊藿苷对COPD模型大鼠超氧化物歧化酶(superoxide dismutase,SOD)活力的影响。方法:将50只雄性SD大鼠随机分为5组,健康对照组(A组),不予处理;COPD模型组(B组)、淫羊藿苷组(C组)、泼尼松组(D组)、淫羊藿苷加泼尼松组(E组),建立大鼠COPD模型并分别给予蒸馏水(10 mL/kg)、淫羊藿苷(100 mg/kg)、泼尼松(5 mg/kg)、淫羊藿苷(50 mg/kg)加泼尼松(2.5 mg/kg)灌胃治疗。在光镜下观察大鼠肺组织病理改变,测定外周血和肺组织匀浆中SOD活力。结果:光镜下A组肺泡结构完整,肺泡壁无炎性细胞浸润;B组肺泡壁及肺间隔炎性细胞浸润,肺间隔断裂、融合,肺实质破坏,形成广泛性肺气肿;C组肺泡壁及肺间隔内炎性细胞浸润明显,肺泡腔扩大,部分出现融合,但肺实质结构破坏程度轻;D组肺组织病理改变近似A组;E组改变介于C组与D组之间,肺泡腔扩大,肺间隔断裂、融合不明显,肺组织炎性浸润明显轻于C组。与A组比较,B组肺组织匀浆及外周血SOD活力显著下降(P<0.01);C、D、E组肺组织匀浆SOD活力下降(P<0.01),外周血中SOD活力呈下降趋势,但差异无统计学意义(P>0.05)。与B组比较,C、E组肺组织匀浆及外周血SOD活力下降较轻(P<0.05);C、E组SOD活力下降趋势相似,差异无统计学意义(P>0.05)。结论:淫羊藿苷能减轻肺组织局部及外周血中的SOD活力的下降,减轻氧化应激对肺组织的损伤。
Objective：To observe the effect of icariin on superoxide dismutase（SOD） activity in a rat model of chronic obstruc- tive pulmonary disease（COPD） induced by cigarette smoking and lipopolysaccharide dripped to trachea. Methods：A total of 50 male adult Sprague-Dawley rats were randomly divided into five groups： control group （Group A）, model group （Group B）, icariin group （Group C）, prednisone group （Group D）, icariin and prednisone group（Group E）. Rats in group A were bred normally. The rest of rats were placed into sealed boxes full of smoke to build rat model of COPD. Before smoking, rats were treated with distilled water at a dose of 10 mL/kg in Group B, icariin at a dose of 100 mg/kg in Group C, prednisone at a dose of 5 mg/kg in Group D, icariin at a dose of 50 mg/kg and prednisone at a dose of 2.5 mg/kg in Group E. The activity of SOD was detected by method of xanthine oxidase. Results： The activity of SOD decreased in serum and lung homogenate of Group B, compared with Group A （P〈0.01）. Pretreatment with icariin or icariin and prednisone could alleviate the sharp decline in SOD activity, compared with Group B（P〈0.05）. There were no significant differences between Group C and Group E（P〉0.05）. Conclusions： Icariin can inhibit the decline in SOD activity in the lung tissues and peripheral blood, and thus alleviate oxidative stress damage in the lung tissues.
出处
《中国临床医学》
2013年第3期249-251,共3页
Chinese Journal of Clinical Medicine
基金
上海市科委自然基金项目(编号:12ZR1418200)
作者简介
通讯作者 李袜群，E-mail：yiqunlee@126．com
