摘要
在甘肃省5个地区采集了100份绵羊血清样本,并对100份样品进行了血清学及病原菌分离培养试验。血清学检测初步鉴定为14份阳性,其中11份来自于同一地区。然后对两例疑似患病羊做病原分离鉴定以及微生物学诊断和PCR鉴定,并对病原菌进行革兰氏染色。本研究成功地从病羊身体中分离出细小、纤细的呈球形、双球形、线状、螺旋状、半月状和短杆状等形态的绵羊肺炎支原体。
One hundred sheep serum samples were collected from five regions of Gansu province and the experiments on serology, pathogen separation and pathogen culture were conducted. There were 14 positive results by serology testing, and 11 of 14 them were from the same area. Two cases of suspected sick sheep MycopLasma pneumoniae were isolated and identified by microbiological diagnosis, PCR and pathogens gram stainingon. The successful separated sheep M. pneumoiane from the sick sheep was small, slender spherical, double spherical, linear, spiral, half-shaped, short rod and other forms.
出处
《草业科学》
CAS
CSCD
北大核心
2013年第9期1428-1432,共5页
Pratacultural Science
基金
甘肃省教育厅基金(1002-06)
关键词
羊支原体肺炎
分离培养
PCR鉴定
sheep Mycoplasma pneumoniae
isolation and culture
PCR identify
作者简介
朱琳（1985-），女，甘肃兰州人，助理工程师，在职硕士生，从事兽用生物制品的研发、生产、经营与技术服务。E—mail：42144704@qq．com
常卫华（1982-），男，山东泰安人，在读博士生，研究方向为I临床兽医学。E-mail：changwe．hua112@163．com
通信作者：赵兴绪（1961-），男，甘肃漳县人，教授，博士，研究方向为兽医产科学及发育生物学。E—mail：zhaoxx@gsau．edu．cn
