摘要
目的:考察不同制备工艺对肺毒清处方药物体外抗病毒作用的影响。方法:采用体外抗病毒法对比肺毒清的水提取物(A)、水醇提取物(D)、水提醇沉物(B)及水提醇沉物的正丁醇萃取物(C)的抗病毒作用。结果:样品D在1 250 mg·L-1下能完全抑制甲1型和甲3型流感病毒,250 mg·L-1样品A在60%细胞毒性下能完全抑制甲1型及甲3型流感病毒,其他样品对其他呼吸道病毒均无抑制作用,且样品D的体外抗病毒作用较样品A更好。结论:相同处方不同制备工艺对复方的药效作用具有一定影响。
Objective: To investigate effects of different preparation processes on in vitro antiviral activity of Feiduqing prescription.Method: Antiviral activities of water extract(A),water and alcohol extract(D),water extraction-ethanol precipitation substance(B) and its n-butanol extract(C) were investigated by in vitro antiviral method.Result: Sample D could completely inhibit A 1 and 3 type influenza viruses with the concentration of 1 250 mg·L-1,sample A with 250 mg·L-1could completely inhibit A 1 and 3 type influenza A viruses under 60% cytotoxicity,other samples had no inhibitory effect on other respiratory viruses,and in vitro antiviral activity of sample D was better than sample A.Conclusion: Different preparation processes of the same prescription had a certain influence of pesticide effect.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第18期42-44,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
成都市科技局高校院所应用成果转化项目(12DXYB020JH-002)
作者简介
刘涛,博士,研究员级高级工程师,从事中成药新药开发及再评价研究,Tel:028-1302236,E-mail:liuta00578@sina.com
[通讯作者]'徐玉玲,高级工程师,从事中成药质量研究及管理研究,Tel:028-61302236,E-mail:xuyuling19750818@sina.com