摘要
目的 探讨肠三叶因子 (ITF)在烧伤后的变化规律及其与肠道损伤和修复的关系。方法 Wis tar大鼠 48只随机分成伤前对照 (C)、烧伤后 (PBD) 1、2、3、5及 7d共 6组。采用高效液相色谱仪定量检测了肠粘膜中ITF的动态变化 ,同时观察了血浆内毒素 (LPS)水平、肠粘膜损伤指数及肠粘膜增殖细胞核抗原(PCNA)的变化 ,并进行相关分析。结果 烧伤后肠杯状细胞分泌ITF的能力下降 ,除伤后第 1天ITF含量有一过性增加外 ,其余各组均明显低于伤前 (P <0 .0 1) ,伤后 7d仅有伤前的 4%。与之对应 ,血浆LPS水平及肠粘膜损伤指数亦大幅上升 ,而肠粘膜PCNA值则明显下降。相关分析显示 ,ITF含量同血浆LPS水平及肠粘膜损伤指数呈显著负相关 ,同肠粘膜PCNA值呈显著正相关。结论 烧伤后肠道合成ITF的能力下降与伤后肠粘膜屏障功能受损。
Objective To explore the principle of intestinal trefoil factor (ITF) expression and its relationship with intestine damage and reparation in rats after severe burns. Methods Forty eight Wistar rats were inflicted with 30% total body surface area full thickness burns and then divided into 6 groups: normal control (C), post burn day 1 (PBD1), PBD2, PBD3, PBD5, and PBD7.The levels of plasma LPS, intestinal proliferating cell nuclear antigen (PCNA) and intestinal mucosa damage index (IMDI) were determined. The content of ITF in the intestinal mucosa was measured with high performance liquid chromatography (HPLC). The relation of the measured results was analyzed. Results The ability of goblet cells to secrete ITF was constantly decreased after burn injury except that the ITF content was increased excessively in PBD1 group. The content of ITF in PBD7 group was just 4% of that in C group. The PCNA level had a remarked decline, but the levels of LPS and IMDI were significantly increased. Correlation analyses showed that the ITF content had a positive correlation to PCNA level but negative correlation to the levels of LPS and IMID. Conclusion The decline of intestinal mucosa to secrete ITF after burn might be associated with the dysfunction of mucosa barrier and the disability of mucosa reparation.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2000年第11期1023-1025,共3页
Journal of Third Military Medical University
基金
全军"九五"攻关项目 !(96L0 4 3)
