摘要
对2010年12月—2011年2月采集自昆明市、曲靖市、普洱市及红河州共6个规模化种猪场的303份疫苗免疫种猪血清及120份21日龄仔猪血清,采用抗体竞争性阻断酶联免疫吸附试验(ELISA)试剂盒检测猪瘟病毒特异性抗体效价。结果发现这些规模化种猪场的猪瘟抗体阳性检出率分别为63.6%(14/22),76.4%(68/89),61.9%(65/105),47.4%(37/78),83.3%(40/48),76.5%(62/81),其中有3个种猪场尚未达到国家猪瘟强制免疫抗体合格率全年应保持在70%以上的要求,提示应及时采取有力措施加强猪瘟免疫工作。针对调查结果,笔者还做了详细的原因分析并提出具体的改进建议。
Antibody-competitive blocking enzyme-linked immunosorbent assay （ELISA） kits were used to detect serum samples of 303 vaccine immunized sows and 120 piglets at day 21, which were collected from 6 scaled breeding pig farms in Kunming, Qujing, Pu＇er and Honghe from December 2010 to February 2011, to determine titers of specific antibodies against classical swine fever virus. As a result, serum positive ratios of the 6 farms were 63.6% （ 14/22）, 76.4% （68/89）, 61.9% （65/ 105）, 47.4% （37/78）, 83.3% （40/48）, 76.5% （62/81）, respectively, 3 of which were lower than a national required percentage of 70% for immunization eligibility, indicating that these breeding pig farms need to enhance immunization against classical swine fever. Detailed discussion about the reasons for the results was made and then specific advice for improvement was put forward.
出处
《云南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2013年第5期755-757,共3页
Journal of Yunnan Agricultural University:Natural Science
基金
云南省现代农业生猪产业技术体系建设资助项目[云财农2009(171)号]
云南省高校科技创新团队支持计划资助项目[云教科(2011)14号]
作者简介
赵永美（1970-），女，云南禄劝人，兽医师，从事动物疫病防治及统计工作。E—mail：fmxyk@126．com
通信作者Correspondingauthor：陈培富（1973-），男，云南弥勒人，博士，副教授，主要从事动物微生物学与免疫学研究。E—mail：cltwins2003@163．com
