期刊文献+

用固定细胞阻断酶联免疫吸附试验鉴别诊断猪传染性胃肠炎病毒和猪呼吸道冠状病毒的感染 被引量:5

Differential Diagnosis of Infections with Swine Transmissible Gastroenteritis virus and Porcine Respiratory coronavirus using fixed cell blocking ELISA
在线阅读 下载PDF
导出
摘要 用固定细胞阻断酶联免疫吸附试验(ELISA)对来自丹麦猪的180份血清进行了猪传染性胃肠炎病毒(TGEV)和猪呼吸道冠状病毒(PRCV)感染的鉴别诊断,共检出了PRCV抗体阳性血清107份(59.4%),TGEV抗体阳性血清0份。同时也检测了一些来自国内不同TGEV感染类型的猪场血清。该鉴别诊断方法在我国的建立和应用为从PRCV阳性国家进口猪的TGE的检疫提供了一条有效途径。 One hundred eighty sera of Denmark imported pigs were differentiated the infections of transmissible gastroenteritis virus of swine(TGEV)and Porcine respiratory coronavirus (PRCV) with the fixed cell blocking ELISA.107 sera of which were found PRCV antibody positive and no TGEV positive serum was found.Some sera from domestic pig farms also were examined in the same test.The extablishment and usage of this technique in our country would provide a effective method for the quarantine of imported pigs from prcv spreaded countries.
出处 《中国兽医杂志》 CAS 北大核心 1997年第12期5-7,共3页 Chinese Journal of Veterinary Medicine
关键词 固定细胞ELISA 鉴别诊断 TGEV PRCV 猪病 fixed cell ELISA differentiation diagnosis TGEV PRCV
  • 相关文献

同被引文献39

  • 1王玉洁,刘金龙,彭树英,杨瑞锋,张涌.应用RT-nested PCR检测猪传染性胃肠炎病毒[J].西北农林科技大学学报(自然科学版),2005,33(2):23-26. 被引量:8
  • 2李丹丹,田志军,姜骞,唐丽杰,李一经.应用TGEV N蛋白McAb间接免疫荧光法检测TGEV的研究[J].中国兽医杂志,2006,42(1):14-15. 被引量:4
  • 3杨群,何孔旺,陆承平.应用多重RT-PCR方法检测158例猪粪样中的两种冠状病毒[J].中国预防兽医学报,2006,28(4):431-435. 被引量:24
  • 4张净,夏谦,高晓燕,王巧全,韩国珍.用阻断酶联免疫吸附试验检测猪传染性胃肠炎病毒抗体和猪呼吸道冠状病毒抗体[J].中国兽医科技,1996,26(12):24-25. 被引量:11
  • 5马思奇.应用免疫荧光直接法检查扁桃体诊断猪传染性胃肠炎[J].中国农业科学,1982,(1):84-88.
  • 6Rim S Y, Song D S, Park B K. Differential detection of transmissible gastroenteritis virus and porcine epidemic diarrhea virus by duplex RT-PCR [J]. J Vet Diagn Invest, 2001, 13(6):516-20.
  • 7Jackwood D J, Kwon H M, Sail L J. Molecular differentiation of transmissible gastroenterisis virus and porcine respiratory coronavirus strains. Correlation with antigenicity and pathogenicity[J]. Adv Exp Med Biol., 1995, 380:35-41.
  • 8Jung K, Kim J, Kim O, et al. Differentiation virus between porcine epidemic diarrhea and transmissible visrus in formalin-fixed gatroenteristis paraffin-embedded tissues by multiplex RT-nested PCR and comparison with in situ hybridization [J]. J Virol Methods, 2003, 108(1):41-47.
  • 9Kim O, Choi C, Kim B, et al. Detection and differentiation of porcine epidemic diarrhea virus and transmissible gastroenteritis virus in clinical samples by multiplex R/-PCR[J]. Vet Rec, 2000, 146(22):637-640.
  • 10Chen R, Huang W, Lin Z, et al. Development of novel real-time RT-PCR assay with LUX primer for the detection of swine transmissible gastroenteritis virus[J]. J Virol Methods, 2004, 122(1):57-61.

引证文献5

二级引证文献24

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部