摘要
目的考察P16与Ki67的表达在子宫颈上皮内瘤变(cervical intraepithelial neoplasia,CIN)组织学诊断与分级中的临床意义。方法针对86例患有宫颈糜烂的育龄期妇女行宫颈组织活检、HE染色常规病理分析及免疫组化SP法检测其P16与Ki67的表达情况。结果 HE染色分析:未见上皮内细胞病变及恶性病变的宫颈组织(NILM)18例(阴性对照组);低级别上皮内瘤变(CINⅠ级)25例,高级别上皮内瘤变(CINⅡ级、CINⅡ～Ⅲ级和CINⅢ级)43例;P16与Ki67免疫组化分析:P16与Ki67在高级别CIN宫颈损伤中的表达明显高于低级别CIN及NILM损伤组织,差异具有统计学意义(P<0.05)。结论 P16与Ki67双阳性标记对于辅助诊断CIN病理分级有益,其可以作为鉴别宫颈癌前病变、指导临床手术治疗及评估预后的生物标识物。
Objective To explore the clinical significance of P16 and Ki67 expression for histological diagnosis and grading of cervical intraepithelial neoplasia （CIN）. Methods A total of 86 cases of cervical erosion in the child-bearing period were detected by cervical biopsy, haematoxylin and eosin （HE） staining, and immunohistochemical SP method for P16 and Ki67 expression. Results HE staining in the tissue samples of cervical biopsy showed that there were 18 specimens of intraepithelial lesion or Malignancy （ NILM）, 25 speci-mens of low-grade CIN lesions （CIN Ⅰ ） and 43 specimens of high -grade CIN lesions （CIN Ⅱ/CIN Ⅱ - Ⅲ/CINⅢ ）. P16 and Ki67 ex-pression was significantly higher in the high-grade CIN lesions than that in the low-grade CIN lesions, non-intraepithelial lesions and ma-lignant lesions of the cervix （NILM） （ P 〈 0.05 ）. Conclusions P16 and Ki67 can be helpful to aid objective CIN lesion grading, which are the diagnostic and prognostic utility and guiding treatment of specific biomarkers in identifying true cancerous precursor lesions.
出处
《安徽医药》
CAS
2013年第9期1540-1542,共3页
Anhui Medical and Pharmaceutical Journal
关键词
宫颈上皮内瘤变
免疫组化
P16
KI67
cervical intraepithelial neoplasia
immunohistochemistry
P16
Ki67
