摘要
目的利用动物模型和生物信息学分析方法对3种外源蛋白(Cry1Ab/Ac、LRP和HSA)的潜在致敏性进行分析。方法动物实验将32只3周龄SPF级雌性Balb/c小鼠分为4组,对照组及Cry1Ab/Ac组、LRP组、HSA组分别以经口灌胃途径给予生理盐水及外源蛋白Cry1Ab/Ac、LRP、HSA,ELISA法测定血清中特异性IgE水平和血浆中组胺水平;利用生物信息学分析方法采用过敏原在线数据库将3种外源蛋白的氨基酸序列与已知过敏原进行对比,判断3种外源蛋白的潜在致敏性。结果动物模型实验结果显示,Cry1Ab/Ac组、LRP组、HSA组小鼠IgE抗体水平分别为(3.83±0.41)、(3.00±0.81)、(3.50±0.55),均高于对照组小鼠的(1.67±0.58),差异均有统计学意义(P<0.05);对照组、Cry1Ab/Ac组、LRP组、HSA组小鼠组胺水平分别为(976.46±179.55)、(1 308.56±465.17)、(985.89±453.54)、(1 149.61±200.97),差异均无统计学意义(P>0.05);生物信息学分析结果显示,HSA和LRP与已知食品过敏原存在同源性,可能存在潜在致敏性,而Cry1Ab/Ac未发现与已知食品过敏原存在同源性。结论 Cry1Ab/Ac、LRP和HSA 3种外源蛋白均具有较强的免疫原性,其中LRP和HSA具有潜在致敏性。
Objective To analyse the potential allergenicity of 3 foreign allergens （insecticidal crystal protein Cry[ Ab/Ac] ）, lysine-rich protein[ LRP], and human serum albumin[ HSA] ）using animal model in vivo and bioinfor- matics analysis. Methods Amino acid sequences of the three foreign proteins were compared with those of the known allergens using online database. Female Balb/c mice were orally exposed to the three foreign proteins. Protein-specific IgE antibodies in serum and plasma histamine levels were detected with enzyme-linked immnnosorbent assay （ELISA）. Results The results of bioinformatics analysis showed that HSA and LRP matched with the known food allergens and had the potential allergenicity, but CrylAb/Ac didn＇t match with the known food allergens. The results of animal model showed that all of the 3 foreign proteins were potentially allergenic. Compared with negative control group, the 3 foreign proteins could increase the level of IgE. The results of histamine detection showed that the three foreign proteins did not significantly increase the histamine level. Conclusion CrylAb/Ac,LRP and HSA have strong immunogenicity and LRP and HSA have potential allergenicity.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2013年第9期1361-1363,共3页
Chinese Journal of Public Health
关键词
外源蛋白
致敏性
动物模型
生物信息学分析
foreign protein
allergenicity
animal model
bioinformatics analysis
作者简介
毕源（1992-），女，辽宁丹东人，本科在读，研究方向：食物过敏。
通讯作者：车会莲，E-mail：chehuilian@cau．edu．cn
