摘要
目的:研究超声在大鼠牙骨质修复过程中对转化生长因子-β1(TGF-β1)表达和骨保护蛋白、核因子-KB受体活化因子配体(OPG/RANKL)表达比值的影响。方法:选择32只健康雄性Wistar大鼠建立正畸性牙根吸收动物模型,随机分为4组,每组8只,分别为空白对照组,单纯加力组,自然修复组,超声修复组,其中用频率1.5MHz,强度100MW/cm2的治疗超声对超声修复组的实验牙在2周的修复期进行每天15min治疗超声照射。2周治疗结束后,取上颌第一磨牙及其牙周组织,制作以上颌第一磨牙为中心的,近远中方向的组织切片,行TGF-β1,OPG,RANKL,免疫组化染色,光镜观察并作免疫组化光密度分析,对实验结果进行单因素方差分析。结果:超声修复组TGF-β1的光密度值(0.36228±0.02833),明显高于自然修复组(0.24576±0.03183),差异有统计学意义。超声修复组OPG/RANKL光密度值比值(1.1126±0.096),也明显高于自然修复组(0.7835±0.067)差异有统计学意义。结论:超声通过上调TGF-β1表达,改变OPG/RANKL的比值,促进牙骨质的修复重建。
Objective： To study the effect of ultrasound on the expressions of TGF-β1 and OPG/RANKL in the rat cementum reconstruction process.Methods： 32 healthy male Wistar rats were established as animal models of orthodontic root resorption,and randomly divided into four groups（the control group,experimental group,natural recovery group,ultrasound repair group） with 8 in each group.The ultrasound repair group were given 1.5MHz 100MW/cm2 ultrasound irradiation for 15min per day in the two weeks of repair period.Upper first molar-centered mesial-distal tissue slices were generated from the upper first molars and peridentium of the rats from each group.Specimen slices were analyzed with hematoxylin-eosin staining,OPG,RANKL,TGF-β1 immunohistochemistry,and optical microscopy.Analyses of densitometry,and one-way analysis of variance were performed.Results： expressions of TGF-β1 densitometry of ultrasound repair group（0.36228 ±0.02833）were significantly higher than natural repair group（0.24576 ±0.03183）.expressions of OPG/RANKL densitometry ratios of ultrasound repair group（1.1126±0.096） were significantly higher than natural repair group（0.7835± 0.067）.Conclusion： ultrasound promoted the repair and the reconstruction of the cementum by increasing the expressions of TGF-β1 and OPG/RANKL ratios.
出处
《口腔颌面修复学杂志》
2013年第1期11-15,共5页
Chinese Journal of Prosthodontics
基金
全军医学科技"十二五"科研项目(项目编号:CWS11J117)
国家自然科学基金青年项目(项目编号:81000417)
作者简介
硕士生
药师
主治医师
医师
徐娟 通讯作者 副主任医师
