摘要
目的:观察实验性牙移动后,初级感觉神经元兴奋性递质降钙素基因相关肽(CGRP)的改变。分析正畸疼痛时,初级感觉神经元痛信号发生、传导及敏感化机制。方法:制备大鼠实验性牙移动动物模型,采用免疫荧光染色法和RT-PCR法分别观察不同时间点三叉神经节内CGRP免疫阳性结构的改变及CGRP mRNA表达的变化。结果:实验性牙移动后,实验侧三叉神经节(TG)内CGRP-ir节细胞以及CGRP mRNA的表达强于对侧。结论:实验性牙移动后,初级感觉神经元中痛感受兴奋性神经递质CGRP的合成、表达增加。表明实验性牙移动影响了初级感觉神经元痛信号的发生、传导,使之致敏。
Objective：To investigate the changes of Calcitonin Gene Related Peptide （CGRP） in the trigeminal ganglion （TG）. To study the effect of the experiment tooth movement on sensitization, conduction of primary sensory neurons and the mechanism of orthodontic pain. Method： Model of the experimental tooth movement was established by insertion of a separating elastic between the upper first and second molars to mimic clinical orthodontic tooth movement and immunohistochemical and RT-PCR methods were used for testing the expressions of CGRP in TG. Result： Comparing to the control side,the number of CGRP-immunoreactive neurons in TG increased in the experimental side;and the gene expression of CGRP mRNA during the experimental tooth movement was upregulated to a different extent and in a timedependent manner. Conclution： During the experimental tooth movement,synthesis of CGRP in primary sensory neurons increases. These results demonstrate that sensibility of primary sensory neurons enhances and that nociceptors are sensitized during the experimental tooth movement.
出处
《临床口腔医学杂志》
2008年第7期396-398,共3页
Journal of Clinical Stomatology
基金
国家自然科学基金资助项目:(30572066)
关键词
实验性牙移动
降钙素基因相关肽
疼痛过敏
experimental tooth movement： calcitonin gene related peptide
hyperalgesia
作者简介
通讯作者：刘洪臣Tel：010-66936254,Email：Liu-he@301dent．com.
