摘要
目的:建立养心达瓦依米西克蜜膏的质量标准。方法:采用TLC法对处方中西红花、肉桂进行定性鉴别,并用RP-HPLC法测定西红花中西红花苷Ⅰ的含量;采用Luna C18(4.6 mm×250 mm,5μm)色谱柱,甲醇-水(45∶55)为流动相,流速1.0 mL.min-1,柱温35℃,检测波长440 nm,进样量10μL。结果:薄层鉴别专属性强,分离效果好。西红花苷Ⅰ在0.019 2～0.153 6μg呈良好的线性关系(r=0.999 9),平均回收率为98.7%(n=6),RSD 1.51%。结论:方法简便、准确、重复性好,可作为养心达瓦依米西克蜜膏的质量控制方法。
Objective： To develop the quality standard of Yangxin Dawayi Mixike Migao.Method： The TLC method was used for the qualitative identification of Stigma Croci and Cortex Cinnamomi in the formula.The content of crocin-I in Yangxin Dawayi Mixike Migao was determined simultaneously by RP-HPLC method with a Luna C18 column（4.6 mm × 250 mm,5 μm）.The mobile phase was methanol-water（45∶ 55）;the flow rate was 1.0 mL.min-1.The detection wavelength was set at 440 nm,and the column temperature was set at 35 ℃.Result： The identification study showed strong specificity.The linear relationship was good.The linear response ranged from 6.04 μg to 45.3 μg for crocin-I and the average recovery was 98.7% with RSD 1.51%.Conclusion： The methods were simple,accurate and with a good reproducibility,and can be used for the quality control of the drug.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第14期108-111,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家药典会国家药品(维药)标准提高项目(799)
作者简介
[通讯作者]沙拉麦提·艾力，副主任药师，从事维吾尔药材及制剂质量研究，Tel：0991-2305956，E-mail：sala001@sina．com
