摘要
目的:建立西红花中西红花苷-1和西红花苷-2的含量测定方法。方法:采用高效液相色谱法,使用Zorbax C_(18)色谱柱,乙腈和水为流动相梯度洗脱,检测波长440nm,流速1.0mL·min^(-1);同时采用单因素分析的方法,对影响含量测定结果的主要因素如提取溶剂、超声处理时间、提取温度及是否避光等进行考察。结果:西红花苷-1在5.2～165μg·mL^(-1),西红花苷-2在2.7～85μg·mL^(-1)范围均呈良好线性关系,相关系数分别为0.9999和0.9998,平均回收率分别为102.5%和98.2%。西红花样品溶液的制备应在避光、室温条件下,以50%乙醇超声处理30min为宜。结论:按照本法进行西红花供试品溶液的制备和含量测定,结果稳定,重现性好,可用于西红花中西红花苷-1和西红花苷-2的含量测定。
Objective： To develop a method for the determination of crocin - Ⅰ and crocin - Ⅱ in stigma of saffron. Method ：An HPLC method was applied with a Zorbax C18 column by gradient elution using acetonitrile and water. The detection wavelength was 440 nm and the flow rate was 1.0 mL ·min^-1; A single factor analysis method was used to evaluate the influence of sample processing conditions, Such as extraction solvents;ultrasonic temperature and time;and whether in the sade;etc. , on the results of content determination. Results：The calibration curve was linear over the concentration range of 5.2 - 165μg· mL^-1 （r =0. 9999）for crocin - Ⅰ and 2.7 - 85 μg·mL^-1 （r =0. 9998）for crocin - Ⅱ. The average recovery of crocin - Ⅰ was 102. 5% and that of crocin - Ⅱ was 98.2%. The sample should be extracted by uhrasonator with 50% ethanol for 30 min, under the room temperature in the shade. Conelusion：A stable and repeatable result can be obtained by the method to prepare sample solution and HPLC conditions. This method can be used for the determination of crocin - Ⅰ and crocin -Ⅱ in stigma of saffron.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2006年第9期1270-1273,共4页
Chinese Journal of Pharmaceutical Analysis
基金
国家"十五"重大科技专项(2001BA701A61)
上海科委中药现代化专项(03D219547
03D219532)资助
作者简介
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