摘要
以神秘果果实为材料,以获得高质量RNA为目的,以针对性地去除多糖、酚类物质为出发点,比较了Trizol法、CTAB法,并相应加以改良。结果显示:CTAB粗提法方便快捷,CTAB精提法提取的总RNA完整性好、纯度高,RT-PCR反应表明提取的总RNA都能满足分子生物学实验的要求。
In order to extract total RNA form miracle fruit and reduce the influence of polysaccharides and polyphenolics, we studied and compared four RNA extraction methods such as Trizol method, Trizol improved method, CTAB crude method and CTAB refined method. The results showed that： The CTAB crude extraction method was convenient and quick, total RAN from the CTAB refined method was proved to be relatively intact and high purity, RT-PCR analysis showed that the total RNA from both CTAB methods could be used in further molecular biology research.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2013年第3期409-412,共4页
Genomics and Applied Biology
基金
"广东高校特色调味品工程技术开发中心"开放课题项目(GCZX-B1103)资助
关键词
神秘果
果实
RNA提取
Miracle fruit, Fruit, RNA isolation
作者简介
通讯作者，tcdeng@126．com
