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基因Ⅳ型戊型肝炎病毒ORF3对靶细胞脂肪因子表达的影响 被引量：2

Effects of Genotype Ⅳ HEV ORF3 on Expression of Adipokine in Target Cells
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摘要 为了探讨戊型肝炎病毒ORF3影响靶细胞脂肪因子表达的分子机制,应用蛋白芯片技术比较稳定表达EGFP-ORF3的HEK293细胞和稳定表达EGFP的HEK293细胞的脂肪因子表达谱,筛选获得显著上调/下调的脂肪因子。结果表明,在稳定表达EGFP-ORF3的HEK293细胞,Fas和TIMP-2的表达水平升高,而ACE-2表达水平降低。研究结果为HEV ORF3对于靶细胞重要脂肪因子表达影响的分子机制研究提供了重要依据。 Human adipokine antibody array was used to compare the expression levels of adipokines in stable cell lines that expressed or did not express SHEV ORF3.The results indicated that the expression of Fas and TIMP2 in ORF3 expressing human embryonic kidney 293 cell line was upregulated,the expression of ACE-2 in ORF3 expressing human embryonic kidney 293 cell line was downregulated,which provide important information for elucidating the molecular mechanism through which HEVORF3 affects the expression of adipokine in target cells.
作者 杜丽 成鹰 史巧芸 焦寒伟 荣辉 张珈宁 贾晓晓 郭莳雨 朱华培 王凤阳
机构地区 海南大学农学院 海南省热带动物繁育与疫病研究重点实验室 海口市动物基因工程重点实验室
出处 《动物医学进展》 CSCD 北大核心 2013年第6期78-80,共3页 Progress In Veterinary Medicine
基金 国家自然科学基金项目(30660139) 海南省社发专项(2012SF016) 海南省自然科学基金项目(311305) 海口市重点科技项目(2010-048)
关键词 蛋白芯片 戊型肝炎病毒 靶细胞 脂肪因子 表达 protein array Hepatitis E virus target cell adipokine expression
分类号 S852.65 [农业科学—基础兽医学]
作者简介 杜丽（1973-），女，山西阳高人，副教授，主要从事功能基因组学研究。 通讯作者
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参考文献14

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二级参考文献25

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共引文献11

同被引文献12

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  • 2Ahmad I,Holla R P,Jameel S.Molecular virology of hepatitis E virus[J].Virus Res,2011,61(1):47-58.
  • 3Wang H,He Y,Shen Q,et al.Complete genome sequence of the genotype 4 hepatitis E virus strain prevalent in swine in Jiangsu province,China,reveals a close relationship with that from the human population in this area[J].J Virol,2012,86(15):8334-8335.
  • 4Dong C,Zafrullah M,Mixson-Hayden T,et al.Suppression of interferon-α signaling by hepatitis E virus[J].Hepatology,2012,55(5):1324-1332.
  • 5Liu T,Lei M,Jiao H,et al.RNA interference induces effective inhibition of mRNA accumulation and protein expression of SHEV ORF3 gene in vitro[J]. Curr Microbiol,2011,62(5):1355-1362.
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二级引证文献4

动物医学进展
2013年 第6期

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