摘要
对花魔芋愈伤组织诱导过程中的多个影响因子进行了研究。试验表明 :以顶芽、侧芽、顶芽鳞片作外植体 ,顶芽鳞片在 10 %NaClO溶液中灭菌 2 5min ,顶芽、侧芽在 0 .1%HgCl2 溶液中灭菌2 0min ,外植体在 10 %柠檬酸溶液或 0 .5 %VC 溶液中切割 ,接种于 1/ 2MS +BA 1mg·l-1+NAA0 .1mg·l-1+4 %蔗糖 +0 .7%琼脂 (pH5 .8)培养基中 ,暗培养 (T :18- 2 0℃ ) ,花魔芋愈伤组织诱导率较高 ,均可达 45 %以上。
In order to increase the callus induction rate of Rivier Giantarum, the influential factors in the process of its callus induction culture are studied. The results are obtained as follows : First, the suitable explants are top buds, lateral buds and bud scales; Second, the sterilization condition of the explants above is that bud scales should be soaked in the 10%NaClO solutions for 25 minutes and top buds or lateral buds in the 0.1%HgCl 2 solutions for 20 minutes.Third,the sterilized explants are soaked and cut in the solutions of 10% citrate or 0.5%V C or their mixture so as to prevent their browning actions. Fourth, the chosen medium is 1/2 MS+BA 1mg·l -1 +NAA 0.1mg·l -1 +4% Sucrose +0.7% agar(pH5.8) . Lastly,the materials are cultured in the darkness (T:18-20℃).$$$$
出处
《湖北民族学院学报（自然科学版）》
CAS
2000年第3期1-3,共3页
Journal of Hubei Minzu University(Natural Science Edition)
关键词
花魔芋
愈伤组织
影响因子
诱导
快速繁殖
Amorphophallus rivieri
Callus induction
Influential factors
