摘要
根据酵母属（ Ｓａｃｃｈａｒｏｍｙｃｅｓ Ｍｅｙｅｎ ｅｘ Ｒｅｅｓｓ） 分类学研究最新进展，核实并更新了保藏于中国普通微生物菌种保藏中心的该属菌株的种类归属。在形态和生理生化性状，包括对６ 种糖的发酵能力、对１８ 种碳源和３ 种氮源化合物的同化能力、在无维生素培养基中和３７ ℃下的生长情况、对放线菌素酮的抗性等常规分类学研究的基础上，对部分疑难菌株进行了脉冲电泳核型比较分析。酿酒酵母（ Ｓａｃｃｈａｒｏｍｙｃｅｓｃｅｒｅｖｉｓｉａｅ） 、贝酵母（ Ｓ．ｂａｙａｎｕｓ） 和巴氏酵母（ Ｓ．ｐａｓｔｏｒｉａｎｕｓ） 三者与少孢酵母（ Ｓ．ｅｘｉｇｕｕｓ） 在电泳核型上具有明显的差异，主要表现在前三者染色体ＤＮＡ 分子的大小范围均为２２５ ～２２００ ｋｂ ，而Ｓ．ｅｘｉｇｕｕｓ 缺少小于３６５ ｋｂ 的染色体ＤＮＡ 分子。Ｓ．ｃｅｒｅｖｉｓｉａｅ 的模式和权威菌株具有１２ ～１４ 条染色体ＤＮＡ 带；Ｓ．ｂａｙａｎｕｓ 和Ｓ．ｐａｓｔｏｒｉａｎｕｓ 的模式菌株均有１７ 条带，但在带型上存在一定差异。原归于Ｓ．ｃｅｒｅｖｉｓｉａｅ 的株菌ＡＳ２－１００ 具有１６ 条带，与Ｓ．ｃｅｒｅｖｉｓｉａｅ 区别明显而与Ｓ．ｂａｙａｎｕｓ 相似；而原定名Ｓ．
The strains of Saccharomyces Meyen ex Reess preserved in China General Microbiological Culture Collection Center (CGMCC) were recharacterized and reidentified according to recent taxonomic improvement of the genus. The strains AS 2 100(originally classified in S.cerevisiae) , AS 2 1158(from former USSR and originally classified in S.exiguus ) and AS 2 1555(from Australia and originally classified in S.uvarum )were found to be different from the standard descriptions of the species concerned in some physiological properties. Comparative CHEF electrophoretic karyotype analysis showed that the chromosomal DNA banding pattern of AS 2 100 was similar to that of the type strain of S.bayanus , while the electrophoretic karyotypes of AS 2 1158 and AS 2 1555 were similar to those of the type and authentic strains of S.cerevisiae. Therefore, AS 2 100 was reidentified as S.bayanus , and AS 2 1158 and AS 2 1555 were reclassified in the speices S.cerevisiae .
出处
《微生物学报》
CAS
CSCD
北大核心
2000年第1期9-13,共5页
Acta Microbiologica Sinica
关键词
酿酒酵母
脉冲电泳核型分析
分类学
Saccharomyces cerevisiae,Saccharomyces bayanus,Electrophoretic karyotype
