摘要
目的:建立布渣叶提取物中牡荆苷、异牡荆苷和水仙苷的含量测定方法。方法:采用Waters XbridgeTMC18色谱柱(4.6 mm×250 mm,5μm),流动相为甲醇-0.1%磷酸水溶液,二元梯度洗脱,流速0.8 mL.min-1,检测波长320 nm,柱温25℃。结果:牡荆苷、异牡荆苷和水仙苷分别在8.512～29.79,8.208～28.73,41.60～145.6 mg.L-1与峰面积呈良好的线性关系,平均加样回收率分别为96.30%,97.32%,102.22%。结论:方法简便、可行,重复性好,结果准确,可作为布渣叶提取物的含量测定方法,为其质量控制提供参考依据。
Objective： To establish a HPLC method for the content determination of vitexin,lsovitexin and narcissin in extract of Microcos paniculata. Method： Waters XbridgeTM C18 （4.6 mm×250 mm, 5 μm） was used with a step gradient of methanol-1%phosphoric acid at a flow rate of 0.8 mL·min^-1The detection wavelength was set at 320 nm, and the column temperature was at 25 ℃. Result： There was good linear relationship in the range of 8.152-29.79 mg·L^-1 forvitexin,8.208-28.73 mg·L^-1 forlsovitexin and 41.60-145.6 mg·L^-1 for narcissin.The average recoveries of vitexin,lsovitexinand narcissinwere 96.30%,97.32%and 102.22% respectively. Conclusion： This method is simple, accurate and can be used for quality control of extract of M.paniculata.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第11期102-104,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广东省中医药局建设中医药强省课题(20111131)
作者简介
[通讯作者]孙冬梅，硕士，主任中药师，从事中药质量评价研究，Tel：020-83482683，E—mail：tcmgdp@163．com
