摘要
背景:成骨细胞体外培养及其中药干预是研究和开发中药治疗骨质疏松症的重要手段之一。目的:观察中药对成骨细胞增殖、分化及对骨钙素合成的影响。方法:收集不同类别中药载药血清对体外成骨细胞及骨钙素分泌影响的相关研究,进行实验数据分析,从细胞分子生物学水平认识传统中药影响成骨细胞合成骨钙素的作用。结果与结论:部分单味中药和中药复方有促进成骨细胞增殖、分化及骨钙素的合成作用,并且不同中药成分对成骨细胞增殖和分化的促进作用及提高骨钙素表达水平不同,与这种中药载药血清浓度在一定范畴内呈正相关。通过对成骨细胞体外培养方法的筛选,找出促进细胞增殖和分化的药物及适宜剂量,对研制治疗骨质疏松症的药物具有积极促进作用。
BACKGROUND： In vitro culture of osteoblasts and its Chinese medicine intervention is one of the important means for the research and development of osteoporosis treated with traditional Chinese medicine. OBJECTIVE： To observe the effect of Chinese medicine on the proliferation and differentiation of osteoblasts and the synthesis of osteocalcin. METHODS： The researches on the effect of different kinds of Chinese medincine-loaded serum on the secretion of osteoblasts and osteocalcin were collected for the data analysis. The effect of traditional Chinese medicine on osteocalcin synthesis of osteoblasts was observed from the level of molecular cell biology. RESULTS AND CONCLUSION： Some single Chinese herbs and Chinese herbal compounds could promote the proliferation and differentiation of osteoblasts and the synthesis of osteocalcin, and different Chinese medicines had different effects in promoting the proliferation and differentiation of osteoblasts and expression level of osteocalcin, which was positively correlated with the concentration of Chinese medicine-loaded serum. Through screening with the in vitro culture of osteoblasts, the drugs and appropriate dose for promoting the proliferation and differentiation were founded, which could positively promote the development of drugs used for the treatment of osteoporosis.
出处
《中国组织工程研究》
CAS
CSCD
2013年第11期2083-2090,共8页
Chinese Journal of Tissue Engineering Research
基金
河北省中医药管理局资助项目(2012068)~~
关键词
组织构建
组织构建学术探讨
中药
成骨细胞
骨钙素
骨质疏松
碱性磷酸酶
体外高糖
羧化不全骨钙素
提取液
载药血清
增殖
骨密度
骨形态蛋白
省级基金
tissue construction
tissue construction academic discussion
Chinese medicine
osteoblasts
osteocalcin
osteoporosis
alkaline phosphatase
in vitro high glucose
carboxylation incomplete osteocalcin
extract
drug-containing serum
proliferation
bone mineral density
bone morphogenetic protein
provincial grants-supported paper
作者简介
魏双平，男，1970年生，河南省安阳县人，汉族，1994年河北医学院毕业，硕士，副教授，主要从事慢性病防治及流行病学的科研及教学研究。xtyzwsp2004@163．com
通讯作者：李瑞玉，教授，主任医师，硕士生导师，邢台医学高等专科学校第二附属医院中西医结合研究所，河北省邢台市054000liruiyu651021@163．com
