摘要
目的研究吉马酮对人肺癌A549细胞增殖及凋亡的影响。方法用MTT比色法检测吉马酮对A549细胞增殖的抑制作用;采用流式细胞仪AnnexinV/PI双染法检测细胞凋亡率;采用电子显微镜观察吉马酮作用细胞后亚细胞结构的变化。结果吉马酮对人肺癌A549细胞的增殖有明显的抑制作用,并呈剂量效应关系;流式细胞仪结果显示,吉马酮可以诱导A549细胞凋亡,凋亡率与药物呈剂量效应关系;电子显微镜下经吉马酮处理后的细胞可见染色质浓聚、染色质边集等典型的凋亡改变,而空白对照组没有变化。结论吉马酮可以抑制肺癌A549细胞的增殖、诱导细胞凋亡。
Objective To study the influence of germacrone on human lung carcinoma A549 cells proliferation and apoptosis. Methods Cell proliferation was measured using the MTT assay. Apoptosis was determined by flow cytometry analysis using an Annexin V-FITC Apoptosis Kit. The changes of nucleus in A549 cells after germacrone-treated were investigated by transmission electron microscopy. Results A549 cells proliferation was suppressed by germacrone in a dose-dependent manner. Flow cytometric analysis showed that germacrone induced A549 cells apoptosis in a dose-dependent manner. Chromosome condensation was observed by electron microscope in treated cells, the untreated cells remained normal. Conclusion Germacrone could inhibit A549 cells proliferation and induce its apoptosis.
出处
《实用药物与临床》
CAS
2013年第4期280-281,共2页
Practical Pharmacy and Clinical Remedies
基金
"盛京自由研究者"基金(201205)
关键词
吉马酮
肺癌
凋亡
Germacrone
Lung cancer
Apoptosis
作者简介
通信作者
