摘要
为了更深入的探讨哈茨木霉菌株T28发酵液乙酸乙酯提取物对致病疫霉生长的抑制作用和机制,采用不同浓度的发酵液乙酸乙酯提取物对致病疫霉进行抑菌试验以及对其体内几种生理代谢酶活性影响进行试验。结果表明:不同浓度的提取物对致病疫霉菌丝生长、孢子囊萌发、孢子囊释放游动孢子均有抑制作用,其中稀释浓度为10倍和100倍时抑制效果较好,均与浓缩液无显著性差异。经提取物处理后致病疫霉菌体48h,与对照相比其保护酶(SOD、CAT、POD、GSH-Px)活性分别下降89.06%,86.96%,97.47%和97.63%,糖代谢酶(HK、PK、LDH)和TCA有氧分解代谢酶(SDH、MDH)分别下降93%,89.56%,88.5%,97.66%和97.43%,能量代谢酶(ATP)活性下降了98.5%和98.67%,MDA含量上升60.98%。研究表明哈茨木霉菌株T28发酵液乙酸乙酯提取物可降低致病疫霉菌体的代谢酶活性,提高菌体MDA含量。提取物处理后致病疫霉菌体遭受损坏,其糖代谢、三羧酸循环、能量代谢等生理活动不能正常进行,这可能是哈茨木霉菌株T28发酵液乙酸乙酯提取物抑制致病疫霉生长的机制之一。
In order to further study the inhibiting activity and mechanism of ethyl acetate extracts of introduced Trichoderma harzianum strain T28 fermentation liquid to the growth of Phytophthora infestans, we investigated bacteriostasis of the extracts in different concentrations on P. infestans and their effects on several enzyme activities in the pest physiological metabolism. The results indicated that all the studied concentrations of the extracts had inhibiting effects on the growth, sporangium germination and spore released zoospores of P. infestans, and among the concentrations the 10 and 100 x diluted had slightly better effects without significant differences. The activities of protective enzymes （SOD, CAT, POD and GSH - Px） of P. infestans treated with the extracts in 48 hours reduced by 89.06% , 86.96% , 97.47% and 97.63%, respectively. Meanwhile, the activities of carbohydrate metabolic enzymes（HK,PK and LDH）and TCA aerobic catabolic enzymes （SDH and MDH）also decreased by 93% , 89.56% , 88.5% , 97.66% and 97.43% , energy - metabolic enzyme activities （ATP） decreased by 98.5% and 98.67%. The MDA contents enhanced 60.98%. This research determined that the ethyl acetate extract from fermentation liquid of T. harzianum strain T28 was able to reduce the metabolic enzyme activities of P. infestans and increase the MDA contents. The extracts could cause the damage to P. infestans and prevent from normal working of its glycometabolism, tricarboxylic acid cycle, energy metabolism and etc, which may be one of inhibiting mechanisms of ethyl acetate extracts of T. harzianum strain T28 on the growth of P. infestans.
出处
《林业科学》
EI
CAS
CSCD
北大核心
2013年第3期110-115,共6页
Scientia Silvae Sinicae
基金
国家林业局"948项目""木霉菌剂制备工艺及保存技术引进"(2009-4-39)
关键词
哈茨木霉菌株T28
提取物
致病疫霉
抑菌机制
Trichoderma harzianum strain T28
extract
Phytophthora infestans
inhibiting mechanism
作者简介
宋瑞清为通讯作者。
