摘要
目的建立高效液相法同时测定复方醋酸棉酚片中维生素B1和B6含量的方法。方法采用C18(150 mm×4.6 mm,5μm)色谱柱;流动相:庚烷磺酸钠溶液(取庚烷磺酸钠0.4 g,加三乙胺3 mL,加水至1 000 mL,用磷酸调pH值至3.5)-甲醇(75∶25);流速:1.0 mL.min-1;检测波长:275 nm。结果维生素B1在10.05～200.92 mg.L-1质量浓度范围内与峰面积呈良好的线性关系Y=13 744.72X+44 526.49(r=0.999 9),回收率为99.4%,RSD=1.1%(n=9)。维生素B6在10.43～208.60 mg.L-1质量浓度范围内与峰面积呈良好的线性关系Y=120101.31X-14766.07(r=0.999 9),回收率100.1%,RSD=1.2%(n=9)。结论该方法简便、准确,可同时测定复方醋酸棉酚片中维生素B1和B6的含量。
Objective To establish an HPLC method for the determination of vitamin B1 and vitamin B6 in compound gosspol acetate tab- lets. Methods The separation was performed on C,s column （150 mm ×4.6 mm,5μm）, and the mobile phase was composed of hep- tanesulfonic acid sodium salt solution （ pH = 3.5 ） ： methanol （ 75 ： 25 ） with the flow rate of 1.0 mL·min^-1 and 275 nm as the detection wave length was set at 275 urn. Results The linear ranges of vitamin B1 and vitamin B6 were 10.05 - 200.92 mg·L^-1（ r = 0. 999 9 ） and 10.43 - 208.60 mg·L^-1 （ r = 0. 999 9）, respectively, and average recoveries were 99.4% （ RSD = 1.1% ） and 100.1% （ RSD = 1. 2% ）, respectively. Conclusion The method was simple and accurate, which can be used for determination of vitamin B1 and vitamin B6 in compound gosspol acetate tablets.
出处
《安徽医药》
CAS
2013年第4期565-566,共2页
Anhui Medical and Pharmaceutical Journal
