摘要
目的：建立高效液相色谱法测定六维胶丸中维生素B1、维生素B2、烟酰胺的含量。方法：采用Agilent 1100高效液相色谱仪，C18。柱（250mm×4．6mm，5μm）为色谱柱；流动相为离子对溶液（含1．5mmol／L戊烷磺酸钠和3．4mmol／L庚烷磺酸钠的0．24％三乙胺溶液，用冰醋酸调节pH值至3．0）-甲醇（80：20），检测波长254nm，流速l，0mL／min。以外标法检测定量。结果：维生素B，的线性范围是4.84～24．2μg/mL，r=0．999674，平均回收率为1．03．3％，RSD=2．0％；维生素B：的线性范围是6．28～31．4μg／mL，r=0．999915，平均回收率为99．5％，RSD=0．7％；烟酰胺的线性范围是35．3～155．32μ/mL，r=0．999917，平均回收率为97．8％，RSD=0．9％。结论：本方法分离度佳、灵敏度高、重现性好、结果可靠。
Objective ：To establish a high-pressure liquid chromatography （HPLC） method for determination of the concentrations of Vitamin B1, vitamin B2, nicotinamide in Liuwei Jiaowan Hexavitamin Soft Capsules. Method： HPLC system （ Agilent 1100 ） was used. The analytical column was packed with C18 （250mm×4.6 mm ID, 5μm）. The mobile phase, consisted of ion pair solution （1.5 mmol/L sodium 1 -pentanesulfonate, 3.4 mmol/L sodium 1 - heptanesulfonate and 0.24% triethylamine, pH = 3.0 adjusted by acetic acid）-methyl alcohol （ 80：20, v/v ）. The detection was operated at 254 nm. Flow rate was set at 1.0 mL/min. The concentrations were quantified by external standard method. Results： The concentration of Vitamin B1 was linear in the range of 4.84-24.2μg/mL （r=0.999 674 ）. The average recovery rate was 103.3% （ RSD=2.0% ）. The concentration of Vitamin B2 was linear in the range of 6.28-31.4μg/mL （ r = 0. 999 915 ）. The average recovery rate was 99.5% （ RSD = 0.7% ）. The concentration of nicotinamide was linear in the range of 35.3-155.32 μg/mL （r=0.999917 ）. The average recovery rate was 97.8%（RSD=0.9%）. Conclusion ： Vitamin B1 , Vitamin B2 and nicotinamide are completely separated under this chromatographic condition. The analytical method shows high sensitivity and good reproducibility. It is proved to be. reliable.
出处
《上海医药》
CAS
2006年第10期474-476,共3页
Shanghai Medical & Pharmaceutical Journal
