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利用改良CTAB法提取卷丹百合鳞叶基因组DNA 被引量:6

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摘要 为了获得高质量卷丹百合鳞叶基因组DNA,在传统CTAB法的基础上进行了改良和优化,筛选出较理想的DNA提取方法。结果表明:用改良CTAB法提取的卷丹百合鳞叶基因组DNA电泳条带清晰,无降解现象,D260 nm/D280 nm为1.6~1.9,用于ISSR-PCR扩增时其稳定性和可重复性都很好,说明DNA纯度和产率完全满足PCR扩增分析的要求。
出处 《江苏农业科学》 北大核心 2013年第3期27-29,共3页 Jiangsu Agricultural Sciences
基金 国家自然科学基金(编号:30860161) 云南省教育厅科学研究基金(编号:2011Y212)
作者简介 陈名红(1974-),男,湖南常德人,博士研究生,副教授,从事植物细胞与分子生物学研究。E—mail:cmhkm@yahoo.com.cn。 通信作者:李成云,博士,教授,从事植物细胞与分子生物学研究。E-mail:li.chengyun@gmail.com。
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参考文献6

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二级参考文献27

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