摘要
为探讨猕猴桃种质资源的遗传多样性,利用SRAP标记对32份猕猴桃种质资源进行遗传多样性分析,并构建其DNA指纹图谱。结果表明,从49对SRAP引物中筛选出12对引物进行PCR扩增,共扩增出191条带,其中多态性条带186条,多态性比率为97.38%。各引物多态性信息含量(PIC)、观测等位基因数(N_a)、有效等位基因数(N_e)、Nei’s基因多样性指数(H)和Shannon’s信息指数(I)的平均值分别为0.893 3、1.969 0、1.400 6、0.221 0和0.387 9,说明32个猕猴桃品种(系)间具有较丰富的遗传多样性。分子方差分析(AMOVA)结果显示:32个猕猴桃种间遗传变异占总变异的51.87%,种内遗传变异占总变异的48.13%。利用UPGMA构建32份猕猴桃种质资源的聚类树状图,在遗传相似系数为0.77处可将32个猕猴桃品种(系)分为4组,聚类结果与猕猴桃传统分类基本一致。利用4对引物扩增的15个多态性位点构建了32个猕猴桃品种(系)的DNA指纹图谱,可以将32个猕猴桃品种(系)区分并准确鉴定。
In order to understand the genetic diversity of Actinidia germplasm,the genetic diversity of 32 Actinidia was analyzed based on SRAP markers,and the DNA fingerprints among them were constructed. The results showed that 12 pair of primers were screened from 49 SRAP primers and 191 SRAP bands were obtained,including 186 polymorphic bands,with a polymorphism rate of 97.38%.The average value of polymorphism information content( PIC),observed number of alleles( N_a),effective number of alleles( N_e),Nei's gene diversity( H) and Shannon's information index( I) were 0.893 3,1.969 0,1.400 6,0.221 0 and 0.387 9,respectively,which indicating that there was comparatively great genetic diversity among 32 Actinidia cultivars. Analysis of molecular variance( AMOVA) indicated that 51.87% of total variability came among species level,while 48.13% came within species level. The cluster analysis conducted with UPGMA showed that 32 Actinidia cultivars were divided into four groups when the genetic similarity coefficient was 0.77,and the constructed phylogenetic trees based on SRAP were consistent with traditional classification of the Actinidia. The DNA fingerprints of 32 Actinidia cultivars were established with 15 sites from four pair of primers, and 32 Actinidia cultivars could be identified by SRAP fingerprints.
出处
《江苏农业学报》
CSCD
北大核心
2018年第1期138-144,共7页
Jiangsu Journal of Agricultural Sciences
基金
中国农业科学院科技创新工程专项经费项目(CAAS-ASTIP-2015-ZFRI)
作者简介
张安世（1965-）,男,河南博爱人,硕士,教授,主要从事植物分子生物学研究.（E-mail）aszhang1212@163.com
