摘要
目的观察羊栖菜多糖(Sargassum fusiforme polysaccharide,SFPS)对糖尿病脑缺血大鼠脑组织细胞凋亡的影响。方法通过HE染色观察SFPS对糖尿病大鼠脑组织形态学改变。通过TUNEL的方法检测脑组织细胞凋亡,并用RT-PCR、免疫组化的方法检测survivin基因mRNA和蛋白的表达的影响。结果SFPS可以明显改善糖尿病脑缺血大鼠脑组织病理形态改变。与模型组相比,SFPS组大鼠脑组织凋亡细胞显著减少,survivin基因的表达明显增加。结论SFPS具有保护糖尿病脑缺血损伤的作用,其作用机制可能与调控survivin基因的表达有关。
Objective To observe the effect of Sargassurn fusiforme polysaccharide（SFPS）on apoptosis of cerebrum tissue after cerebral ischemia injury in diabetic rats. Methods HE was used to stain hippocam- pal slices. Apoptosis was measured in cerebrum tissue with TUNEL method. Protein expression of Survivin was detected with immunochistochemistry and RT- PCR was used to measure rnRNA experes- sion. Results SFPS could improve the cerebrum diabetic model group, the number of apoptotic pathologic changes of diabetic rats. With comparison to cells in cerebrum tissue decreased and Survivin expres- sion increased in treated group. Conclusions SFPS could relieve brain damage induced by cerebra ische mia injury in diabetic rats, which may be related to regulating Survivin expression.
出处
《中国海洋药物》
CAS
CSCD
北大核心
2013年第2期63-66,共4页
Chinese Journal of Marine Drugs
基金
黑龙江省教育厅科学技术研究项目(11511427)
牡丹江医学院科学研究技术项目(2011-08)资助
关键词
羊栖菜多糖
糖尿病
细胞凋亡
Sargassum fusiforme polysaecharide
diabetes
cell apoptosis
作者简介
关悦（1984-），女，主治医师，研究方向：糖尿病及其慢性并发症的相关性研究，E-mail：guanyuemdj@126．com
通讯作者：李扬，男，主治医师，研究方向：糖尿病及其慢性并发症的相关性研究，E-mail：liyangmdj@163．com
