摘要
目的观察电针(EA)老年痴呆症大鼠海马CaMKⅡ表达的的关系。方法 SD雌鼠24只,随机分为假手术组(n=8),模型组(n=8),电针组(n=8),模型组及电针组用切除卵巢颈背部皮下注射D-半乳糖的方法造成记忆损伤的动物模型。电针组电针"百会"-"四神聪"穴,1次/d,连续10 d。采用Morris水迷宫法测试动物记忆行为变化。用免疫组化法测定海马CAMKⅡ阳性产物的表达。结果与模型组比较,电针组大鼠逃避潜伏期显著缩小(P〈0.05);与模型组比较,电针组海马CA1区CaMKⅡ表达明显上调。结论电针"百会"-"四神聪"穴后可使海马CA1区CaMKⅡ表达明显上调,改善了大鼠学习记忆的能力。
Objective To observe the effect of electroacupunoture （EA） to the expression of Calcium/calmodulin - dependent protein kinase Ⅱ （ CAMK Ⅱ ） in rat hippocampus tissues in senil dementia rats. Methods A total of 24 SD rats were randomized into control, Sham, OVX and OVX ＋ EA groups ,with 8 cases in each. For rats of the OVX and OVX ＋ EA groups, subcutaneous injection of D -galactose was given once daily for 7 W. EA（2/15Hz, 1 -2 mA） was applied to ＂baihui＂ - ＂sishengcong＂, for 30min, once daily Morris water maze test was conducted to evaluate the rats＇memory - learning ability. CAMK Ⅱ expression of hip- pocampus was detected by immunohistochemistry. Results Compared with Sham group, escape latency shorten significantly after EA （P 〈 0.05 ） ; CAMK Ⅱ immunoreaction positive product integral grey values （IGV） in rat hippocampus of OVX ＋ EA groups were significantly lower （ upregulation of expression） than OVX group （ P 〈 0.05 ）. Conclusion EA ＂baihui＂ - ＂sishengcong＂ has an effect To improve the ability of learning and memory in rats which is closely related to the upregulation of CAMK Ⅱ expression.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2013年第1期236-238,共3页
Lishizhen Medicine and Materia Medica Research
基金
海南省自然科学基金(No.310054)
海南省卫生厅课题(琼卫2010-45)
海南医学院人才引进启动基金(No.2010008)
作者简介
徐秋玲（1978-），女（汉族），辽宁沈阳人，现任海南医学院中医学院副教授，博士学位，主要从事针刺镇痛及改善记忆的机理研究工作，
通讯作者：刘涛（1977-），男（汉族），辽宁沈阳人，现任海南医学院中医学院副教授，博士学位，主要从事动物证候相关研究工作．
