摘要
为建立一种快速测定牛奶中新霉素的新方法,以市售超高温灭菌纯牛奶为样品,基于样品中残留新霉素与酸性品红在pH5.50的B-R(Britton-Robinson)缓冲溶液中形成新的缔合物,利用共振光散射技术进行测定。结果表明:其最大共振光散射峰位于625nm波长处,在0.00～2.50μg/mL范围内新霉素质量浓度与体系的相对散射光强度(I)有良好的线性关系,相关系数r=0.9978,检出限为0.013μg/mL;样品测定的RSD小于2.5%(n=5),加标回收率为84.0%～96.7%。此法快速简便、灵敏度较高,用于牛奶中新霉素残留量的测定,结果满意。
In order to explore a novel method for the rapid determination of neomycin residues in milk, ultra high temperature （UHT）-sterilized pure milk from supermarket was used as the sample to determine neomycin residues in milk on basis of the reaction between neomycin and acidic fuchsin in Britton-Robinson buffer at pH 5.50 through resonance light scattering （RLS） technology. Results indicated that the maximum RLS peak was observed at 625 nm. A good liner relationship （r = 0.9978） was observed in chlorpyrifos concentration range of 0.00-2.50 μg/mL and the detection limit of neomycin was 0.013 μg/mL. The relative standard deviations （RSD） of the precision tests for 5 repeated determinations of all samples were less than 2.5%. The recovery rate of this developed method was in the range of 84.0%-96.7%. Due to simple operation, rapid determination and high sensitivity, this developed method is suitable for the determination of neomycin residues in milk.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第6期219-222,共4页
Food Science
基金
河南省重点学科建设项目(10466-X-082301)
关键词
共振光散射
新霉素
酸性品红
牛奶
resonance light scattering
neomycim acidic fuchsin
milk
作者简介
高向阳（1949-），男，教授，主要从事食品分析、食品资源开发研究。E—mail：ndgaoxy@163．com
