摘要
用超声薄膜分散法制备丹参酮脂质体,结合正交试验和单因素法筛选丹参酮脂质体的配方,通过高速离心和高低温试验研究丹参酮脂质体的稳定性;通过颗粒大小、含量和包封率等探究脂质体的理化性质;用高效液相色谱法检测丹参酮脂质体的含量;用场透射电子显微镜观察丹参酮脂质体的形貌特征。结果表明,丹参酮脂质体的优选处方质量比为:大豆卵磷脂∶胆固醇=4∶1,卵磷脂∶丹参=20∶1,水合介质为去离子水,温度20℃。测量其粒径为800 nm～1 000 nm,滤膜挤压后粒径为200 nm～300 nm,包封率达82%。丹参酮脂质体在不同温度下保存3个月,粒径含量基本无变化。实验证明此方法制备的丹参酮脂质体稳定性好,包封率高,有助于丹参酮在化妆品等日用化学领域的应用。
F ilm-uhrasonic wave method is used to prepare the Tanshinone liposome. Tanshinone liposome formula is screened by both orthogonal test and single factor method. Centrifugation and temperature cycling test are used to test the stability of Tanshinone liposome ; the particle size, content and encapsulating rate are determined to characterize the physico-chemical property of Tanshinone liposome ; high performance liquid chromatography （HPLC） method is used to measure the content of Tanshinone liposome, and the morphology of Tanshinone liposome is observed by transmission electron microscope. The results indicated that the weight ratios of the optimized formulation were lecithin： cholesterol=4：1 and lecithin： Tanshinone=20：1 ; deionized water was used as hydration medium, and the operating temperature was at 20℃. The liposome particle size was 600 nm ~ 800 nm typically; moreover, with the help of membrane filter, it can be reduced to 200 nm ~ 300 nm, while the entrapment efficiency reached 82% at the same time. The particle size kept constant at different temperature for 3 months. It has been proven that Tanshinone liposome has very good stability and highly encapsulating rate which contribute to daily use of Tanshinone in chemical field such as cosmetics.
出处
《日用化学品科学》
CAS
2013年第2期28-32,共5页
Detergent & Cosmetics
基金
国际合作项目(2008DFB50060)
作者简介
作者简介：郭星木（1988-），女，山东人，硕士。
