摘要
建立了应用高效液相色谱-二极管阵列检测器(HPLC-DAD)测定卷烟主流烟气和滤嘴中茄尼醇的方法,测定了21个牌号卷烟样品烟气中茄尼醇的释放量,并研究了滤嘴截留茄尼醇量与卷烟主流烟气中茄尼醇、烟碱、焦油释放量的相关性。结果表明:①采用本方法在7 min内即可完成对茄尼醇的检测,在0.5～100.0μg/mL范围内线性良好(R2=1),定量限为0.47μg/支,加标回收率为94.28%～106.70%,相对标准偏差(RSD)为2.06%;②不同牌号卷烟主流烟气中茄尼醇释放量差别较大,为34.47～300.00μg/支;③滤嘴截留茄尼醇量与卷烟主流烟气茄尼醇、烟碱、焦油的R2分别为0.9967,0.9467和0.9954。该方法适合于卷烟主流烟气中茄尼醇的快速分析,可为吸烟者烟气主要有害成分暴露量评估方法的建立提供参考。
A method for determining the solanesol in mainstream cigarette smoke （MCS） and filter with high performance liquid chromatography-diode array detector （HPLC-DAD） was established, the deliveries of solanesol in MCS of 21 cigarette brands were determined, and the correlation between the amount of solanesol retained by cigarette filter and the deliveries of solanesol, nicotine and tar in MCS was researched. The results showed that： 1） Using the established method, the analysis of solanesol could be finished within 7 minutes. The linear range of the method was from 0.5 to 100.0 μg/mL with the correlation coefficient （R2） of 1. The limit of quantification （LOQ） was 0.47 μg/cig., and the recoveries ranged from 94.28% to 106.70% with the relative standard deviation （RSD） of 2.06%. 2） The deliveries of solanesol in MCS of the tested brands ranged from 34.47 to 300.00 μg/cig. 3） The correlation coefficients （R2） between the amount of solanesol retained by cigarette filter and the deliveries of solanesol, nicotine and tar in MCS were 0.9967, 0.9467 and 0.9954, respectively. This method is suitable for the rapid determination of solanesol in MCS, which provides a reference for establishing an assessment method for the exposure of main harmful components in MCS to smokers.
出处
《烟草科技》
EI
CAS
北大核心
2013年第1期46-49,共4页
Tobacco Science & Technology
基金
郑州烟草研究院科技项目"卷烟烟气多生物标志物检测研究"(322010CZ0590)
郑州烟草研究院科技项目"基于滤嘴分析的吸烟者烟气主要有害成分暴露量评估方法研究"(312011CZ0590)
作者简介
陈玉松（1985-），硕士，助理工程师，主要从事烟草化学研究。E-mail：cys0227@126．com
通讯作者：刘惠民E-mail：liuhuimin63@x263．net
