摘要
研制了石墨烯与L-胱氨酸复合膜化学修饰电极(GR-L-CysS/GCE),并研究了盐酸异丙肾上腺素在修饰电极上的电化学行为和测定方法。结果表明,在0.2 mol/L Na2HPO4-柠檬酸(pH 7.4)溶液中,GR-L-CysS/GCE对盐酸异丙肾上腺素的电化学氧化具有明显的催化增敏作用,氧化峰电流相对于在裸玻碳电极上增加了13倍。在优化实验条件下,定量测定的线性范围为4.0×10-6～1.6×10-4mol/L,r为0.9977,方法检出限(S/N=3)为8.4×10-7mol/L。探讨了盐酸异丙肾上腺素在GR-L-CysS/GCE上的电催化过程和反应机理,测得在本体系中参与反应的电子转移数和质子数均为1,电子转移系数为0.4635。对样品进行测定及加标回收实验,回收率在94.9%～102.9%之间。
A graphene-L-cystine modified glassy carbon electrode（GR-L-CysS/GCE） has been prepared.The electrochemical behavior of isoprenaline hydrochloride at the modified electrode has been studied.In 0.2 mol/L Na2HPO4-citric acid（pH 7.4） buffer,GR-L-CysS/GCE exhibited excellent catalytic and enhancement effect on the electrochemical oxidation of isoprenaline hydrochloride,and the oxidation peak current increased ca.13 times compared with that at bare GCE.The measurement parameters were optimized.Under the optimal conditions,isoprenaline hydrochloride concentration was linear with peak current in the range of 4.0×10-6-1.6×10-4 mol/L（R=0.9977）,and the detection limit was 8.4×10-7 mol/L（S/N=3）.The mechanism investigation showed that the reaction of isoprenaline hydrochloride at the modified electrode was a process with one-proton and one-electron transfer,and the coefficient of electron transfer α is 0.4635.This method was successfully used in the determination of isoprenaline hydrochloride in injection with the recovery between 94.9%-102.9%.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2012年第12期1877-1882,共6页
Chinese Journal of Analytical Chemistry
基金
广西自然科学基金(No.2010GXNSFA013001)资助项目
关键词
石墨烯
L-胱氨酸
化学修饰电极
盐酸异丙肾上腺素
电化学行为
Graphene
L-Cystine
Chemically modified electrode
Isoprenaline hydrochloride
Electrochemical behavior
作者简介
Email：zhuocai@gxu．edu．cn
