摘要
利用合成的环糊精聚合物与亚甲基蓝间的超分子作用及与戊二醛的缩聚作用，将介体和酶共同固定在电极上。制成的过氧化氢传感器有良好的稳定性和充分的电子流动性，在pH6．80的磷酸盐缓冲溶液，100mV／s的扫速条件下，以-0．8-0．4V范围内用循环伏安法对过氧化氢溶液进行扫描测定分析。实验结果显示，还原峰电流与过氧化氢浓度在4．0×10^-6～2．5×10^-3mol／L范围内成良好的线性关系，相关系数达0．9984，最低检出限为5．0×10^-7mol／L。用于样品测定，效果良好。
A polymeric β-cyclodextrin is prepared, the polymers and methylene blue with horse radish peroxidase enzyme formed a adoptive film-modified glassy carfbon electrode, its electrochemical behavior towards the oxidation of hydrogen peroxide is investigated. The Experimental results show that polymer modified electrode could reduce the oxidation overpotential of hydrogen peroxide with the increasing of value of pH6.80 of phosphate buffer solution, the peak current of oxidation is proportional to the concentration of hydrogen peroxide and a good linear relation from 4.0 × 10^-7 to 2.5 × 10^-3 mol/L（ r =0. 9984） with a detection limit of 5.0 × 10^-7mol/L is obtained. The methods have been applied to determine hydrogen peroxide in samples, satisfactory results are obtained.
出处
《东华理工学院学报》
2006年第4期383-386,共4页
Journal of East China Institute of Technology
关键词
Β-环糊精聚合物
修饰电极
电化学
β-cyclodextrin polymer
modified electrode
electrochemistry
作者简介
刘淑娟（1973-），女，硕士，主要从事分析化学方面的教学与研究。
